Collagenase P
ROCHE/COLLP-RO - from Clostridium histolyticum
Product Type: Chemical
| application(s) | life science and biopharma |
| biological source | Clostridium histolyticum |
| collagenase activity | >1.5 U/mg (Lyophilizate, Collagenase activity) |
| color | light brown to brown |
| concentration | 0.5-2.5 mg/mL |
| foreign activity | Clostripain 24.099 U/mg |
| Protease 4.4 U/mg (Proteases) | |
| Trypsin 53.428 U/mg (with BAEE) | |
| form | lyophilized |
| manufacturer/tradename | Roche |
| NCBI accession no. | WP_138210331   |
| packaging | pkg of 1 g (11249002001) |
| pkg of 100 mg (11213857001) | |
| pkg of 2.5 g (11213873001) | |
| pkg of 500 mg (11213865001) | |
| Quality Level | 100 |
| solubility | water: soluble |
| sterility | non-sterile |
| storage temp. | 2-8°C |
| technique(s) | tissue processing: suitable |
| Application: | Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Because of its high collagenase activity Collagenase P is particularly suitable for the isolation of pancreatic islets from mouse and rat (function tested). The preparation is also suitable for the isolation of adipocytes from epididymal fat pads of rats. |
| Biochem/physiol Actions: | |
| Features and Benefits: | Contents: Lyophilizate, nonsterile |
| General description: | Collagenase P is an enzyme mixture prepared from the extracellular culture filtrate of a special Clostridium histolyticum strain.Collagenase P is a research biochemical, not manufactured under the US good manufacturing practices (GMP) regulations. Note: Collagenase P is a non-sterile preparation of the culture supernatant of Clostridium histolyticum. It is neither designed nor intended for isolation of pancreatic islets for transplantation into humans and must not be used for such purposes. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Activator: Ca2+ Inhibitors: Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol Collagenase is not inhibited by serum. Clostripain inhibitors: TLCK Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean) Working concentration: 0.5 to 2.5mg/ml 0.5 to 1.5mg/ml for the isolation of pancreatic islets from mouse and rat pancreas. Approximately 2mg/ml for the isolation of adipocytes. Storage conditions (working solution): -15 to -25°C The reconstituted solution is stable at -15 to -25°C. Prepare appropriate aliquots and avoid repeated freezing and thawing. |
| Preparation Note: | Reconstitution in any balanced salt solution (e.g., HBSS) |
| Preparation Note: | Store dry |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | collagenase activity: >1.5 U/mg (Lyophilizate, Collagenase activity) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.24.3 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |