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CDP-Star®

ROCHE/CDP-RO - Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate

Synonym: CDP-Star® Chemiluminescent Substrate; ready-to-use, cdp-star; Disodium 2-chloro-5-(4-methoxyspiro[1,2-dioxetane-3,2′-(5-chlorotricyclo[3.3.1.13.7]decan])-4-yl]-1-phenyl phosphate

CAS Number: 160081-62-9
Empirical Formula (Hill Notation): C18H19Cl2O7Na2P
Molecular Weight: 495.20
MDL Number: MFCD02094611
Linear Formula: C18H19Cl2O7Na2P
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-11685627001 1 mL
$383.00
1/EA
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45-11759051001 1 mL
$652.00
1/EA
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CDP-Star, ready-to-use

 

assay >98%
form solution
InChI 1S/C18H21Cl2O7P.2Na/c1-24-18(14-6-13(2-3-15(14)19)25-28(21,22)23)17(26-27-18)11-4-10-5-12(17)9-16(20,7-10)8-11;;/h2-3,6,10-12H,4-5,7-9H2,1H3,(H2,21,22,23);;/q;2*+1/p-2/t10-,11-,12+,16-,17?,18?;;
InChI key UKWLRLAKGMZXJC-VCCHNUGISA-L
manufacturer/tradename Roche
packaging bottle of 1 mL (11685627001[25 mM])
  bottle of 2 × 1 mL (11759051001[25 mM])
Quality Level 100 
shipped in wet ice
SMILES string Cl[C@]1(C2)C[C@H]3C4(OOC4(C5=CC(OP([O-])([O-])=O)=C(Cl)C=C5)OC)[C@@H]2C[C@@H](C1)C3
storage temp. 2-8°C
Application: Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:
• Southern blot
• Northern blot
• Dot blot
• Colony and plaque hybridization
• Gel shift assays
Features and Benefits: CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.
• Save time with a fast, ultra-sensitive substrate.
• Easily strip and reprobe membranes.
• Take multiple exposures of up to two days for each experiment.
• Reuse the substrate by filtering and storing in sodium azide.
General description: Contents
25 mM solution (12.38 mg/ml), 100x concentration
Legal Information: CDP-Star is a registered trademark of Tropix, Inc.
Other Notes: • First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.
• No preincubation step required.
• Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).
• Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
• Exposure times are shorter than with CSPD.
• Do not use nitrocellulose membranes.
• Repeat exposures can be made up to two days after the addition of substrate.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C
Quality: Purity: Purified by HPLC.
RIDADR NONH for all modes of transport
WGK Germany nwg
Flash Point(F) No data available
Flash Point(C) No data available
Purity >98%
Storage Temp. 2-8°C
UNSPSC 12352200

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