β-Galactose Dehydrogenase
ROCHE/BGALD-RO - from Pseudomonas fluorescens
Synonym: D-galactose dehydrogenase; D-
Product Type: Chemical
| biological source | Pseudomonas fluorescens |
| color | white |
| foreign activity | ADH <0.01% |
| galactosidase <0.01% | |
| LDH <0.5% | |
| NADH oxidase <0.1% | |
| form | suspension |
| manufacturer/tradename | Roche |
| mol wt | 64,000 Da |
| optimum pH | 8.4 (0.1 M in Tris buffer) |
| packaging | pkg of 1 mL (10104973001 [1 mg]) |
| pkg of 1 mL (10104981001 [5 mg]) | |
| pH range | 4.6-9.2 |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| solubility | water: soluble |
| specific activity | ~5 units/mg protein (At 25 °C with D-galactose as the substrate; standardized with BSA.) |
| storage temp. | 2-8°C |
| suitability | suitable for enzyme test |
| technique(s) | activity assay: suitable |
| UniProt accession no. | (T1WEA6 · T1WEA6_PSEFL)  |
| Analysis Note: | Contaminants: <0.01% ADH, <0.01% ?-galactosidase, <0.1% NADH oxidase, <0.5% LDH |
| Application: | β-Galactose Dehydrogenase has been used as a reporter enzyme in neuraminidase (NA) kinetic parameters for the natural substrates 6′-Sialyl-N-acetyllactosa |
| Biochem/physiol Actions: | Galactose dehydrogenase will oxidize D-galactose [Km = 0.7 mM (30 °C, pH 9.1); relative rate = 1.00], L-arabinose (relative rate = 0.47), D-fucose (relative rate = 1.05), 2-deoxy-D-galactose (relative rate = 0.27), 2-amino-2-deoxy-D-galacto Galactose dehydrogenase does not oxidize L-galactose, D-arabinose, D-glucose, L-fucose, D-ribose, D-xylose, D-glucuronic acid, D-galacturonic acid or D-galactose-6-phosphate. The enzyme will use either NAD (Km = 0.24 mM; relative rate = 1.0) or NADP (Km = 2.3 mM; relative rate = 0.32). |
| General description: | β-Galactose Dehydrogenase plays a crucial role in galactose metabolism by catalyzing the pyridine-nucleotide-depen |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Other Notes: | Galactose dehydrogenase from Pseudomonas fluorescens (MW 64,000 D) is a dimer with subunits of equal size (subunit MW = 32,000 D). |
| Other Notes: | One unit galactose dehydrogenase will oxidize 1 mol of β-D-galactose in one minute at +25 °C and pH 8.6. The QC assay produces 1 mol of NADH per mol of galactose oxidized. |
| Physical form: | Suspension in 3.2 M ammonium sulfate solution, 1 mM EDTA, pH approximately 6 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| activity | specific activity: ~5 units/mg protein (At 25 °C with D-galactose as the substrate; standardized with BSA.) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.48 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |