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BCIP

ROCHE/BCIPP-RO - 99% (HPLC), powder, solubility: in DMF, soluble

Synonym: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt; BCIP® p-toluidine salt; X-phosphate p-toluidine salt

CAS Number: 6578-06-9
Empirical Formula (Hill Notation): C8H6BrClNO4P · C7H9N
Molecular Weight: 433.62
MDL Number: MFCD00040636
Linear Formula: C8H6BrClNO4P · C7H9N
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-10760994001 250 mg
 $212.00
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45-11585002001 1 g
 $462.00
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assay 99% (HPLC)
description 5-bromo-4-chloro-3-indolyl-phosphate, 4-toluidine salt
form powder
InChI 1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
InChI key QEIFSLUFHRCVQL-UHFFFAOYSA-N
manufacturer/tradename Roche
mol wt Mr 326.44 (BCIP)
  Mr 433.6 (BCIP toluidine salt)
packaging pkg of 1 g (11585002001)
  pkg of 250 mg (10760994001)
Quality Level 100 
SMILES string Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
solubility DMF: soluble
storage temp. 2-8°C
Analysis Note: Absorption: Absorption maximum in the visible light is not determined.
Analysis Note: Performance test: incubation with AP
Application: For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Application: For the colorimetric detection of alkaline phosphatase-labeled molecules.
General description: BCIP serves as substrate for alkaline phosphatase. The reaction product has a blue color and is insoluble in water. The blue color can be visually detected.
General description: Histochemical substrate for alkaline phosphatase.
Legal Information: BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes: For life science research only. Not for use in diagnostic procedures.
Purity 99% (HPLC)
Storage Temp. 2-8°C
UNSPSC 12352204

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