Acetyl-Coenzyme A
ROCHE/ACOA-RO - 85% (Enzymatic and Absorbance), 2% (lithium)
Synonym: acetyl-coa
Product Type: Chemical
| assay | 2% (lithium) |
| 85% (Enzymatic and Absorbance) | |
| description | Chemical Formula: C23H35N7O17P3SLi3 |
| form | solid |
| manufacturer/tradename | Roche |
| mol wt | Mr 809.6 (acetyl-CoA) |
| Mr 827.4 (acetyl-CoA-Li3) | |
| packaging | pkg of 010 mg (10101893001) |
| pkg of 050 mg (10101907001) | |
| pkg of 200 mg (11585371001) | |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | Acetyl-Coenzyme A is used to assay CAT enzyme activity in cell extracts using radioisotopes. CAT enzyme activity in cell extracts catalyzes the transfer of acetyl groups from acetylcoenzyme A to chloramphenicol. A number of assays have been developed to measure CAT activity in cell extracts. Acetyl-Coenzyme A has also been used to determine citrate synthase activity. |
| Biochem/physiol Actions: | Acetyl-Coenzyme A (Ac-CoA) is the end product of glycolysis and takes part in the Ac-CoA pathway, which is a metabolic pathway for carbon compounds. Ac-CoA is important in cholesterol synthesis. It also takes part in fatty acid biosynthesis and catabolism of polyamines like spermine and spermidine. A low level of Ac-CoA leads to a loss in glial cell and neuronal function. Ketone bodies and triglycerides give rise to Ac-CoA on hydrolysis and this indirectly leads to increased histone acetylation. |
| General description: | Acetyl-Coenzyme A, trilithium salt |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working solution: Optimal solvent: water or aqueous solution with a weak acidic pH (4 to 5). Storage conditions (working solution): -15 to -25 °C 50 mg/ml in phosphate buffer, pH 7, is stable for 3 weeks at -15 to -25 °C. Unfrozen solutions should be immediately used. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | 2% (lithium); 85% (Enzymatic and Absorbance) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |