Lumi-Light Western Blotting Substrate
ROCHE/12015200001
Synonym: western blotting substrate
Product Type: Chemical
Detect as little as 50 pg. Different amounts of Β-galactosidase were separated by SDS-PAGE, 6% acrylamide, and then blotted onto a PVDF membrane. After incubation with Anti-Β-galactosidase monoclonal antibody (3 μg/mL), horseradish peroxidase-labeled mouse antibody (100 mU/mL) was used as a secondary detection reagent. Detection was performed for 10 minutes using the Lumi-Imager Instrument.
Time-response curve of a Lumi-Light signal (in Boehringer Light Units) from a typical western blot, analyzed on the Lumi-Imager Instrument.
| manufacturer/tradename | Roche |
| packaging | pkg of 400 mL |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | Lumi-Light Western Blotting Substrate has been used in western blotting. |
| Application: | Lumi-Light Western Blotting Substrate is a chemiluminescent POD substrate for western blots. Routine detection of any antigen blotted on a PVDF or nitrocellulose membrane. It is suited for high-sensitivity routine western blotting, especially when quantification is required. The chemiluminescent signal persists for more than 3 hours, allowing multiple exposures to be taken. |
| Features and Benefits: | • Detect rare proteins: Lumi-Light substrate detects antigen in the range of 10 - 50pg. • Multiple exposures possible: The signal is stable for more than three hours after substrate addition. • Save sample material: Lumi-Light substrate detects less than 50-pg amounts of antigen. • Save primary antibody: Due to the strong signal of the Lumi-Light substrate, primary antibody can be diluted up to 10-fold more than with colorimetric detection systems. • Easy preparation of substrate solution: Just mix the two Lumi-Light components in a ratio of 1:1. Contents Lumi-Light Luminol/Enhancer Solution, 2 x 100ml Lumi-Light Stable Peroxide Solution, 2 x 100ml |
| General description: | As the technology for quantifying chemiluminescence advances, the substrates for chemiluminescence must also advance. For instance, the light emission of conventional enhanced luminol substrates substantially decreases within 30 minutes. In contrast, the chemiluminescence signal of the Lumi-Light substrate persists for more than three hours, and since exposures of only a few minutes are necessary, multiple exposures can be taken. The emission wavelength of the Lumi-Light substrate is 425nm. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 2 components |
| Quality: | Each lot is function tested using mouse or rabbit primary antibodies. |
| Specifications: | Number of Tests: The reagent is sufficient for 40 blots, each with a size of 10cm x 10cm. Sensitivity: Depending on the affinity of the primary antibody, 10 - 50pg of antigen can be detected |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |
| Components | Lumi-Light Luminol/Enhancer Solution; Lumi-Light Stable Peroxide Solution |