Anti-HA-Fluorescein, High Affinity
ROCHE/11988506001 - from rat IgG1
Synonym: antibody
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 3F10, monoclonal |
| conjugate | fluorescein conjugate |
| epitope sequence | YPYDVPDYA |
| form | lyophilized |
| isotype | IgG1 |
| manufacturer/tradename | Roche |
| packaging | pkg of 25 μg |
| Quality Level | 100  |
| storage temp. | −20°C |
| Application: | Anti-HA-Fluorescein, High Affinity has been used in immunofluorescence labeling of chinese hamster ovary live cells and in immunocytochemistry of neuron cell culture. |
| General description: | Anti-HA-Fluorescein, High Affinity is a monoclonal antibody for the highly sensitive detection of HA-tagged recombinant proteins, Fab fragments, conjugated to fluorescein. Anti-HA-Fluorescein, High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the human hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging". Contents Lyophilizate, stabilized |
| Immunogen: | Amino acids 98 to 106 from the human influenza virus hemagglutinin protein |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Stabilizers: proteinous stabilizers Working concentration: Flow cytometry: 1 to 5 μg/ml Immunofluorescence: 1 to 5 μg/ml Working concentration of conjugate depends on application and substrate. Concentrations should be taken as a guideline. Storage conditions (working solution): Reconstituted antibody is stable at 2 to 8 °C for two months. Alternatively store aliquots at -15 to -25 °C for up to six months. Note: Avoid thawing and repeated freezing. |
| Quality: | The Anti-HA-Fluorescein, High Affinity antibody is function tested by flow cytometry analysis of a HA-tagged fusion protein |
| Reconstitution: | Add 0.5 ml double-distilled water to a final concentration of 50 μg/ml. Rehydrate for 10 minutes prior to use. |
| Specificity: | Anti-HA-Fluorescein, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P273 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |