MIA ELISA
ROCHE/11976826001 - sufficient for ≤96 tests, Roche, kit of 1 (9 components), suitable for ELISA
Synonym: ELISA
Product Type: Chemical
manufacturer/tradename | Roche |
packaging | kit of 1 (9 components) |
Quality Level | 100 |
storage temp. | 2-8°C |
technique(s) | ELISA: suitable |
usage | sufficient for ≤96 tests |
Application: | For research use only. Not for use in diagnostic procedures. The MIA ELISA is an ideal application in research studies determining the melanoma inhibitory activity protein in serum and plasma. The MIA ELISA is specially developed for the quantitative in vitro determination of MIA protein in serum and plasma within streptavidin-coated microplates (MPs). It has a wide applictaion in MIA (melanoma inhibitory activity) ELISA assays. |
General description: | One-step immunoreaction—photometri Standards: Six standards, ranging from 0 to 50 ng/ml MIA protein, are provided with lot-specific data. |
Immunogen: | Detailed epitope structure is not known. The epitopes recognized by the antibodies are conformation epitopes, no linear structures. This is why the abs are not suitable for Western (denaturation destroys epitopes). The two antibodies recognize different epitopes (non-overlapping). The antibodies recognize non-overlapping epitopes, which are conformational epitopes (no linear sequence is recognized but rather parts of 3D-conformation). Exact epitopes have not been determined. |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Packaging: | 1 kit containing 9 components. |
Preparation Note: | Working solution: Beside the ready-to-use solutions supplied with this kit, you will need to prepare the following working solution:
Roche recommends using only double distilled water for the reconstitution of lyophylizates. Storage conditions (working solution): Anti-MIA-Biotin (vial 1): 6 months at -15 to -25 °C or ≤ -60 °C when aliquoted and shock-frozen Anti-MIA-Peroxidase (vial 2): 6 months at -15 to -25 °C or ≤ -60 °C when aliquoted and shock-frozen MIA-Standard (vial 3): 1 day at 2 to 8 °C or 6 months at -15 to -25 °C or ≤ -60 °C when aliquoted and shock-frozen MIA-Control Serum (vial 4): 1 day at 2 to 8 °C or 6 months at -15 to -25 °C or ≤ -60 °C when aliquoted and shock-frozen Washing Buffer (vial 6): 1month at 2 to 8 °C Immunoreagent: 8 hours at 2 to 8 °C |
Specificity: | The MIA ELISA detects natural and recombinant human melanoma inhibitory activity protein. No cross-reaction with other serum components has been found. |
Symbol | GHS07,GHS09 |
Signal word | Warning |
Hazard statements | H317 - H411 |
Precautionary statements | P261 - P273 - P280 - P333 + P313 - P362 + P364 - P391 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
Storage Temp. | 2-8°C |
UNSPSC | 12352200 |
Components | Anti-MIA-biotin antibody, monoclonal from mouse, lyophilized (clone 2.F7.3B1); Anti-MIA-peroxidase antibody, monoclonal from mouse, lyophilized (clone 1.A12.9A1); MIA Standard, lyophilized; MIA Control Serum, MIA in human serum, lyophilized approximately 25 ng/ml; Incubation Buffer ready-to-use; Washing Buffer; ABTS Substrate Solution ready-to-use; Streptavidin-coated Microplate modules; Self-adhesive Plate Cover Foils |