TUNEL Dilution Buffer
ROCHE/11966006001 - pH 7.2-7.4 (20 °C), suitable for detection, pkg of 2 × 10 mL
Synonym: TUNEL; transferase dUTP nick end labeling
Product Type: Chemical
| application(s) | detection |
| manufacturer/tradename | Roche |
| packaging | pkg of 2 × 10 mL |
| pH | 7.2-7.4 (20 °C) |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | TUNEL dilution buffer has been used in terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays. |
| Application: | TUNEL Dilution Buffer has been used: • to dilute terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) enzyme (TdT) in preparing TUNEL reaction mixture for TUNEL critical commercial assays • to quantify apoptosis in rat kidney • to dilute the terminal deoxynucleotidyl transferase enzyme solution to identify DNA fragmentation in oligodendrocytes in vitro • for TUNEL assays |
| General description: | The TUNEL dilution buffer can be used in combination with the in-situ cell death detection kits. It can be used to dilute terminal deoxynucleotidyl transferase (TdT) for the TUNEL reaction in special applications and also to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: • ELISA: 100 mU/ml • Western blot: 100mU/ml Storage conditions (working solution): Dilutions of TdT should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H350i - H412 |
| Precautionary statements | P201 - P202 - P273 - P280 - P308 + P313 - P501 |
| RIDADR | UN 1556 6.1 / PGIII |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 41116100 |