Titan One Tube RT-PCR Kit
ROCHE/11939823001 - sufficient for 50 reactions, suitable for RT-PCR
Synonym: 1 step RT-PCR
Product Type: Chemical
| feature | dNTPs included: no |
| hotstart: no | |
| input | purified RNA |
| manufacturer/tradename | Roche |
| parameter | 42-60 °C optimum reaction temp. |
| Quality Level | 100  |
| storage temp. | −20°C |
| technique(s) | RT-PCR: suitable |
| usage | sufficient for 50 reactions |
| Application: | Titan One Tube RT-PCR Kit has been used: • to complete cDNA synthesis in reverse transcription-quantitativ • to clone sirtuin 6 (SIRT6) into the backbone of pDsRed2-N1 • to amplify full-length and truncated cDNA fragments of C-X-C motif chemokine 5 (CXCL5) • to analyze RNA (total RNA, poly (A)+ RNA, viral RNA) from a variety of sources (animal, plant, human, viral, and bacterial). In a single tube, the Titan One Tube RT-PCR Kit will reverse transcribe RNA into cDNA,† then amplify the cDNA with a thermal cycling reaction. The enzymes AMV Reverse Transcriptase and the Expand High Fidelity PCR System are used, respectively. Depending on the primers chosen, a specific message or the entire population of transcripts can be amplified. The RT-PCR method also permits the cloning of rare messages without the need to construct cDNA libraries. The kit may be used for:Qualitative, semi quantitative, or quantitative analysis of RNA transcription levels even in single cells in combination with gel-based detection methods or ELISA-based detection methods • for cloning of RNA up to 6 kb • for mutation analysis at RNA level in combination with sequencing or other mutation scanning techniques |
| Features and Benefits: | • Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparison to Taq DNA polymerase. • Delivers increased sensitivity due to the high efficiency of all three enzymes. Is faster than conventional two-step RT-PCR. |
| General description: | The Titan One Tube RT-PCR Kit is designed for fast, sensitive and reproducible analysis of RNA by high fidelity reverse transcription polymerase chain reaction (RT-PCR) in a one-step reaction. |
| General description: | Titan™ One Tube RT-PCR Kit uses Avian Myeloblastosis Virus (AMV) reverse transcriptase for first strand cDNA synthesis and the Expand™ High Fidelity enzyme blend consisting of Taq DNA polymerase and Tgo DNA polymerase for amplification of cDNA by PCR. Tgo DNA polymerase possesses proofreading activity leading to an increased PCR fidelity. This kit reduces the error rate, allows the amplification of fragments up to 6 kb by RT-PCR, delivers increased sensitivity. It is considered faster than conventional two-step RT-PCR. |
| Legal Information: | Expand is a trademark of Roche |
| Legal Information: | Titan is a trademark of Roche |
| Legal Information: | Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under such patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 9 components |
| Specificity: | Specificity of the reaction strongly depends on primer design and DNA contamination. To exclude artefacts from DNA targets appropriate positive and negative control reaction should be included. Heat inactivation: 2 min at 94 °C Reverse Transcriptase AMV |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 - H412 |
| Precautionary statements | P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 41106313 |
| Components | Titan Enzyme Mix; dNTP Mix, mixture of dATP, dCTP, dGTP, and dTTP solution 10 mM; RNase Inhibitor 5 U/μl; Human Control RNA 2 pg/μl; Control Primer Mix 20 μM; Water, PCR Grade; RT-PCR Reaction Buffer 5x concentrated; DTT Solution 100 mM; MgCl2 Stock Solution 25 mM |