Anti-His6
ROCHE/11922416001 - from mouse IgG1
Synonym: metal chelate affinity chromatography
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | monoclonal |
| conjugate | unconjugated |
| form | lyophilized |
| isotype | IgG1 |
| manufacturer/tradename | Roche |
| packaging | pkg of 100 μg |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | Anti-His6 is used for the detection of His6-tagged proteins in: • Immunoblotting (e.g., dot blots and western blots) • Immunoprecipitation • Immunoassays (ELISA) • Immunocytochemistry • Immunofluorescence |
| General description: | Anti-His6 is a monoclonal antibody to His6-tagged proteins. Anti-His6 specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the location of the histidine-tag in the protein sequence. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Stabilizers: BSA Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: • ELISA: for detection 0.1 μg/ml; for coating 1 to 5 μg/ml • Immunoprecipitation: 0.5 to 5 μg/ml • Western blot: 0.2 to 0.5 μg/ml |
| Reconstitution: | Add 1 ml double-distilled water to a final concentration of 100 μg/ml. |
| Specificity: | Anti-His6 specifically recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the histidine-tag in the protein sequence. In addition, Anti-His6 preferentially recognizes the C-terminal His6-epitope with high sensitivity. Anti-His6 allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used. |
| Specificity: | The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |