DNA Molecular Weight Marker XVI (250 bp ladder)
ROCHE/11855638001 - pkg of 50 μg (in 200 μl), solution
Synonym: DNA marker
Product Type: Chemical
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 50 μg (in 200 μl) |
| Quality Level | 100  |
| storage temp. | −20°C |
| Application: | DNA Molecular Weight Marker XVI (250 bp ladder) may be used as a molecular weight marker in agarose gel electrophoresis: • for the molecular weight comparison of random amplified polymorphic DNA (RAPD) amplified fragments of plant genomic DNA and meat products • for enterobacterial repetitive intergenic consensus (ERIC) products generated from polymerase chain reaction • in reverse transcription-polymerase chain reaction (RT-PCR) amplified dsRNA sequences of African horse sickness virus |
| General description: | Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases. Size Range: 0.25 to 3.09 kbp |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Ready-to-use solution, 250 μg/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). |
| Preparation Note: | Storage conditions (working solution): 2 to 8 °C Once thawed, store at 2 to 8 °C. |
| Sequence: | The mixture contains 12 blunt-ended, double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 250, 500, 750, 1000, 1250, 1500, 1750, 2000, 2250, 2500, 2750, and 3000. The 1000- and 2000-bp banding pattern are 3 times brighter. Electrophoretic separation of this molecular weight marker results in a regular pattern. Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. The fragments exhibit uniform spacing (e.g., on a 0.7% agarose gel) for easier reading, while bands 1000 and 2000bp are 3 times brighter for easy orientation. |
| Unit Definition: | 50 μg = 1A260 unit |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 41105335 |