Annexin-V-FLUOS
ROCHE/11828681001 - solution, suitable for flow cytometry
Product Type: Chemical
Discrimination between apoptotic and necrotic U937 cells treated with camptothecin. Late-stage apoptosis detected with Annexin-V-FLUOS (green), and counterstained with propidium iodide (necrotic cells).
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 500 μL |
| Quality Level | 100  |
| shipped in | dry ice |
| storage temp. | −20°C |
| technique(s) | flow cytometry: suitable |
| usage | sufficient for 250 tests |
| Application: | In the early stages of apoptosis, changes occur at the cell surface. One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner part of the plasma membrane to the outer layer, by which PS becomes exposed at the external surface of the cell. Annexin-V-FLUOS detects apoptotic cells in suspension cells using flow cytometry (FACS) or fluorescence microscopy, by binding to phosphatidylserine on the outer leaflet of apoptotic cell membranes. A simultaneous labeling with, e.g., propidium iodide (PI) is needed for differentiation of apoptotic from necrotic cells. Other secondary labeling is possible, e.g., membrane surface staining with a phycoerythrin or TRITC-labeled monoclonal antibody for further cellular characterization. |
| General description: | Annexin-V-FLUOS is a phospholipid-binding protein with a high affinity for phosphatidylserine (PS). Detection of cell-surface PS with annexin-V thus serves as a marker for apoptotic cells. Analysis may be by flow cytometry or by fluorescence microscopy. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Amount of rGFP recommended for: • SDS-PAGE (Coomassie stained): 1μg • Western blot: 5ng Storage conditions (working solution): Diluted staining solution should be always prepared freshly. Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system. Sample material: Cell lines and freshly isolated cells. |
| Specificity: | Annexin-V binds in a Ca2+-dependent manner to negatively charged phospholipid surfaces, and shows high affinity for phosphatidylserine. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |