Anti-Protein C Affinity Matrix
ROCHE/11815024001 - from mouse IgG1κ
Synonym: affinity matrix, anti-protein c
Product Type: Chemical
| antibody form | purified immunoglobulin |
| biological source | mouse |
| capacity | 2-10 nmol/mL binding capacity |
| clone | HPC4, monoclonal |
| conjugate | agarose conjugate |
| form | slurry |
| isotype | IgG1, kappa |
| manufacturer/tradename | Roche |
| packaging | pkg of 1 mL (settled resin volume) |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Analysis Note: | KD = 10–9 M Binding Capacity is 2 to 10 nmol/ml affinity matrix. Yield of 10.5 nmol purified protein/ml affinity matrix was determined using a whole-cell bacterial extract containing protein C-tagged β-galactosidase. |
| Application: | Anti-Protein C Affinity Matrix is used for: • Immunoprecipitation of Protein C-tagged proteins from mammalian, bacterial, and yeast cell extracts • Affinity column purification of Protein C-tagged proteins from crude protein extracts Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by: • Western blotting using the Anti-Protein C antibody • Silver staining (or similar protein stain) |
| Features and Benefits: | • use gentle elution conditions using calcium-chelating agents like EDTA. • highly specific to EDQVDPRLIDGK, derived from protein C. • binding of Anti-Protein C to protein C-epitop is dependent on the presence of calcium ions. • suitable for purification of proteins containing protein C as N-terminal, C-terminal or internal fusion. • applicable with crued cell extracts from mammalian, bacterial, and yeast expression systems. Contents 1. The antibody is covalently coupled to agarose beads and supplied as a 2ml slurry containing 1ml beads and 1ml buffer. 2. 4mg of antibody is reacted per ml of beads in the coupling reaction. 3. A plastic column with top and bottom caps is included. |
| General description: | Protein C is a Vitamin K-dependent plasma zymogen that is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme. Anti-Protein C mouse monoclonal antibody (clone HPC4) binds specifically to an epitope sequence spanning the thrombin cleavage site of protein C and is immobilized. Anti-Protein C recognizes the 12-amino acid sequence (EDQVDPRLIDGK), which encodes residues 6 through 17 of the heavy chain of Protein C. The formation of the Anti-Protein C/protein C epitope complex is dependent on the presence of calcium ions. In the presence of Ca2+, the antibody binds with high affinity and specificity to this sequence in native human Protein C or in proteins tagged with this epitope. This efficient binding within the recombinant fusion protein occurs regardless of the site of incorporation of the epitope tag (i.e., N terminus, C terminus, or within the reading frame). This unique antibody is especially well suited for purification of recombinant fusion proteins tagged with the protein C epitope. • Insertion of the protein C tag does not introduce a new metal-ion binding site. The antibody contains the Ca2+ binding site. • Protein C tag can be integrated either at the N-terminus, C-terminus or internally without any change in antibody specificity. • Rapid immunoaffinity purification under non-denaturing conditions using economical calcium chelating agent (e.g., EDTA) or alternatively a specific protein C-tag peptide. Monoclonal mouse antibody Anti-Protein C (clone HPC4) is covalently coupled to agarose beads. In the coupling reaction 4mg of antibody is reacted per 1ml of beads. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing settled resin and column |
| Physical form: | 1 ml settled resin of Anti-Protein C Affinity Matrix in 20 mM Tris, 0.1 M NaCl, 1 mM CaCl2, and 0.09% sodium azide (w/v); 2 ml suspension equals to 1 ml bed volume. One plastic column with top and bottom caps is included |
| Quality: | Each lot of Anti-Protein C Affinity Matrix is tested for its ability to purify a Protein C-tagged protein expressed in transformed bacteria from crude bacterial extract. The antibody affinity column is used in combination with western blot and/or silver stain analysis. |
| Specificity: | Anti-Protein C recognizes the 12-amino acid sequence EDQVDPRLIDGK, which encodes residues 6 to 17 of the heavy chain of protein C. In the presence of Ca2, the antibody binds with high affinity and specificity to this sequence in native human protein C or in proteins tagged with this epitope. Efficient binding within the recombinant fusion protein occurs regardless of epitope position (N-terminal, C-terminal, or internal). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |