rGFP
ROCHE/11814524001 - Green Fluorescent Protein from jellyfish Aequorea victoria, recombinant expressed in E. coli
Synonym: gfp, r-; green fluorescent protein
Product Type: Chemical
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 50 μL (1 mg/ml) |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Application: | Recombinant green fluorescent protein (rGFP) has also been used in indirect enzyme-linked immunosorbent assay. |
| Application: | Recombinant Green Fluorescent Protein (rGFP) is suitable as: • Positive control when detecting GFP fusion proteins by SDS-PAGE. • Positive control when detecting GFP fusion proteins by western blot using Anti-GFP monoclonal antibody. • Standard for experiments involving fluorescence microscopy. |
| General description: | GFP isolated from jellyfish Aequorea Victoria exhibited spontaneous fluorescence property. It is a novel important marker for gene expression in various heterologous systems. It is stably present in plant cells and shows photobleaching. Molecular cloning of GFP produces a fused protein with fluorescence excitation and emission spectra. |
| Other Notes: | Emission max: 509nm Excitation max: 395nm |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution, 1 mg/ml rGFP in 5 mM Tris-HCl, 5 mM EDTA, pH 8.0 |
| Preparation Note: | Working concentration: Amount of rGFP recommended for: • SDS-PAGE (Coomassie stained): 1 μg • Western blot: 5 ng |
| Specificity: | Heat inactivation: Fluorescence will be quenched by reducing reagents, such as DTT or sodiumdithiosulfate, and is completely inhibited by peroxide. Fluorescence is inhibited by extreme pH conditions (pH < 4 and pH > 11) as well as ethanol content higher than 60%. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Storage Temp. | 2-8°C |
| UNSPSC | 23201100 |