mini Quick Spin RNA Columns
ROCHE/11814427001 - pkg of 50 columns, Roche
Synonym: spin column
Product Type: Chemical
Removal of unincorporated nucleotides from 5′ end-labeled oligomers with the mini Quick Spin DNA and Oligo Columns. Various oligomers, ranging from 8 to 32 bases, were labeled with gamma-32P-ATP via the 5′ End-Labeling Kit. Unincorporated nucleotides were removed from the samples with either mini Quick Spin DNA or mini Quick Spin Oligo Columns. One fifth of each isolated sample was electrophoresed through a 20% acrylamide gel in TBE buffer (electrophoresis for 5-6 hours at 150 V). After electrophoresis, the gel was overlayed with plastic wrap and exposed to Lumi-Film for approximately 18 hours. Lanes 1: Labeled oligos, unpurified 2, 3: Labeled oligos, purified with the mini Quick Spin DNA Column 4: Blank 5: Labeled oligos, unpurified 6, 7: Labeled oligos, purified with the mini Quick Spin Oligo Column 8: Blank 9: Labeled oligos, unpurified Result: The mini Quick Spin Columns clearly removed unincorporated nucleotides. The gel also shows that mini Quick Spin Oligo Columns should be used to purify oligonucleotides that are smaller than 20 base pairs. (Compare lanes 2 and 3 with lanes 6 and 7).
Preparing the column
Purifying the sample
| manufacturer/tradename | Roche |
| packaging | pkg of 50 columns |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | mini Quick Spin RNA Columns has been used in RNA in situ probe production, critical commercial assays and mRNA purification. |
| Application: | Ready-to-use, disposable, microfuge-compatible columns designed for the removal of unincorporated radiolabeled nucleotides from RNA labeled by polymerase or end-labeling techniques. Use the highly purified RNA in northern blotting. The mini Quick Spin columns are designed for use with variable-speed, fixed-angle microcentrifuges. |
| Features and Benefits: | • Save time and effort with ready-to-use columns. • Purify more nucleic acid in less time - process up to 75μl of sample in only 7 minutes. • Minimize sample loss while maximizing yield - short and effective two-step procedure. • Easy-to-use - column and cap allow for rapid resuspension of the gel matrix, and the bottom tip can be quickly snapped off. • Ensure optimal performance and reproducibility with quality tested columns: High recovery of radiolabeled RNA Maximum retention of unincorporated nucleotides Absence of RNases Contents Pre-packed, pre-swollen, microfuge-compatible columns with a cap and snap-off bottom tip. Suspension of Sephadex G-50 in STE buffer (10mM Tris-HCI, pH 7.5, 1mM EDTA, 100mM NaCl). |
| General description: | Ready-to-use, microcentrifuge-compatibl |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Sample size: 20 – 75μl Time required: 7 minutes Reactions/standard sample: 50 |
| Principle: | The mini Quick Spin gel filtration columns separate molecules based on their relative size. This rapid chromatographic separation can be done in a conventional tabletop microcentrifuge. During centrifugation, mini Quick Spin Columns allow larger molecules (DNA, RNA, or oligonucleotides) to pass through quickly while retaining smaller molecules, such as unincorporated nucleotides. |
| Quality: | RNases are not detected, according to the current Quality Control procedures. |
| Specifications: | Recovery of labeled RNA: ≥80% Retention of unincorporated nucleotides: >99% |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 41115700 |