mini Quick Spin Oligo Columns
ROCHE/11814397001
Synonym: spin column
Product Type: Chemical
Removal of unincorporated nucleotides from 5′ end-labeled oligomers with the mini Quick Spin DNA and Oligo Columns .
| manufacturer/tradename | Roche |
| packaging | pkg of 50 columns |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Application: | Mini quick spin oligo columns has been used to remove unincorporated radioactive nucleotides from the labeled oligo substrates. |
| Application: | Ready-to-use, disposable, microfuge-compatible columns designed for the removal of unincorporated radiolabeled nucleotides from end-labeled oligonucleotides. The mini Quick Spin columns are designed for use with variable-speed, fixed-angle microcentrifuges. |
| Application: | The mini Quick Spin columns are used for quick and complete removal of unincorporated nucleotides (e.g., radionucleotides or fluorescent dye-labeled dideoxy terminators) from labeled nucleic acids that have been prepared by nick translation, end labeling, polymerization, or other labeling techniques. Use the highly purified DNA in Southern blotting and sequencing. |
| Features and Benefits: | Ready-to-use disposable columns designed to quickly and efficiently remove unincorporated nucleotides from freshly prepared radiolabeled oligonucleotides (≥8 bases). mini Quick Spin Columns can be used in any standard microcentrifuge. • Save time and effort with ready-to-use columns with ready-to-use columns. • Purify more nucleic acid in less time - process up to 50μl of sample in only 7 minutes. • Minimize sample loss while maximizing yield - short and effective two-step procedure. • Easy-to-use - column and cap allow for rapid resuspension of the gel matrix, and the bottom tip can be quickly snapped off. • Ensure optimal performance and reproducibility with quality tested columns: a. High recovery of radiolabeled oligos b. Maximum retention of unincorporated nucleotides c. Absence of DNases |
| General description: | Ready-to-use, disposable, microfuge-compatible chromatography columns designed for the removal of unincorporated radiolabeled nucleotides from end-labeled oligonucleotides. The mini Quick Spin columns are designed for use with variable-speed, fixed-angle microcentrifuges. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | Pre-packed, pre-swollen, microfuge-compatible columns, with a cap and snap-off bottom tip. |
| Physical form: | Suspension of Sephadex G-25 in STE buffer (10mM Tris-HCI, pH 7.5, 1mM EDTA, 100mM NaCl). |
| Principle: | The mini Quick Spin gel filtration columns separate molecules based on their relative size. This rapid chromatographic separation may be done in a conventional tabletop microcentrifuge. During centrifugation, mini Quick Spin columns allow larger molecules (DNA, RNA, or oligonucleotides) to pass through quickly while retaining smaller molecules, such as unincorporated nucleotides. |
| Specifications: | Recovery of labeled oligonucleotides: ≥80% Retention of unincorporated nucleotides: >95% Sample size: 20 – 50μl Time required: 7minutes Reactions/standard sample: 50 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 41115700 |