Anti-c-myc-Peroxidase
ROCHE/11814150001 - from mouse IgG1κ;
Synonym: anti-myc-peroxidase
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| assay | 90% (HPLC and SDS-PAGE) |
| biological source | mouse |
| clone | 9E10, monoclonal |
| conjugate | peroxidase conjugate |
| epitope sequence | EQKLISEEDL |
| form | solution |
| isotype | IgG1κ |
| manufacturer/tradename | Roche |
| packaging | pkg of 500 μg (500 μl) |
| Quality Level | 100  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | • Use Anti-c-myc-Peroxidase for the detection of native human c-myc protein and recombinant epitope-tagged proteins that contain the c-myc epitope using: Dot blots • Western blots |
| General description: | Anti-c-myc-Peroxidase is a monoclonal antibody to c-myc peptide (clone 9E10), conjugated to horseradish peroxidase. Anti-c-myc recognizes the 9E10 epitope (EQKLISEEDL), derived from the human c-myc protein. The monoclonal antibody against the c-myc epitope is well characterized and does not cross-react with other cellular proteins. The antibody recognizes its antigenic determinant even when the c-myc-peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging". |
| Immunogen: | Amino acid sequence EQKLISEEDL was derived from the human c-myc protein. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: • Western blot: 1 μg/ml |
| Quality: | Function test: Western blot using a cell line that expresses a recombinant c-myc-tagged protein. |
| Specificity: | Anti-c-myc-Peroxidase recognizes the 9E10 epitope (EQKLISEEDL), which was derived from the human c-myc protein. The monoclonal antibody against the c-myc epitope is well characterized and does not crossreact with other cellular proteins. The antibody recognizes its antigenic determinant even when the c-myc peptide epitope is introduced into unrelated recombinant proteins either N-terminal, C-terminal or internal. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Purity | 90% (HPLC and SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |