β-Gal Reporter Gene Assay, chemiluminescent
ROCHE/11758241001 - sufficient for 500 assays (microplate), sufficient for 250 assays (tubes)
Synonym: galactosidase
Product Type: Chemical
Test principle
Kinetic of light reaction under reporter gene assay conditions.
| manufacturer/tradename | Roche |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| usage | sufficient for 250 assays (tubes) |
| sufficient for 500 assays (microplate) |
| Application: | The β-Gal Reporter Gene Assay, chemiluminescent, is used to quantitatively measure β-Gal expression in eukaryotic cells that are transfected with a plasmid bearing the β-Gal-encoding lacZ reporter gene. |
| Features and Benefits: | • Sensitive: Chemiluminescence technology allows detection of approximately 20 fg β-galactosidase in cell extracts • High dynamic measuring range: Linear range over four to five orders of magnitude • Constant light emission: The assay produces a long-lasting light emission instead of a short-peak kinetic • Safe: No radioactive isotopes are used • Fast: Approximately 1.5 to 2.5 hours elapse from start to finish • Convenient: Kit contains all reagents needed, including lysis buffer, protease inhibitors, and a positive control |
| General description: | The β-Gal Reporter Gene Assay is designed for specifically measuring bacterial β-galactosidase activity. To achieve this, the enzyme reaction is conducted at a pH that is optimized for bacterial β-Gal, but allows no significant endogenous eukaryotic β-galactosidase activity. However, if very high endogenous β-galactosidase activity is found (e.g., with hepatic cells), heat treatment can be performed to ensure the specific determination of the amount of bacterial β-galactosidase that is encoded by the transfected plasmid. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 7 components. |
| Preparation Note: | Storage conditions (working solution): Substrate Reagent (solution 1) is stable at least for 24 h at 2 to 8 °C. Initation Reagent (solution 2) is stable for at least 4 weeks when stored at 2 to 8 °C. Lysis Reagent, 1x (solution 3) Lysis reagent without protease inhibitors is stable at -15 to -25 °C or for 3 months at 2 to 8 °C. Lysis reagent containing protease inhibitors is stable for one week when stored frozen at -15 to -25 °C. Positive Control (solution 4) The reconstituted enzyme solution is stable for 1 week at 2 to 8 °C. When stored frozen in aliquots at -15 to -25 °C the enzyme is stable for 3 months. Avoid multiple freezing and thawing. |
| Symbol |    GHS02,GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H225 - H290 - H314 - H317 - H336 - H412 |
| Precautionary statements | P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P370 + P378 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 2 |
| Storage Temp. | 2-8°C |
| UNSPSC | 23201100 |
| Components | β-Gal Substrate, Galacton Plus 100x concentrated; Assay Buffer; Enhancer; Initiation Solution; Protease Inhibitors; Lysis Buffer 5x concentrated; Positive Control, β-galactosidase (E. coli), lyophilizate |