mRNA Isolation Kit
ROCHE/11741985001
Synonym: mRNA isolation; nucleic acid isolation
Product Type: Chemical
Figure 1: Detection of ACE-specific mRNA in chicken eye tissue by RT-PCR
Typical total RNA/mRNA Recovery
Principle
| manufacturer/tradename | Roche |
| packaging | kit of for isolation of at least 70 μg of poly (A)+ RNA |
| Quality Level | 100  |
| Analysis Note: | Typical Sample* Cultured cells: 2 x 107 Tissue: 200 mg Total RNA: 500 μg * Larger or smaller samples may also be used. Time Required: Approximately 30 minutes Product: Poly(A)+ RNA, in double-distilled water |
| Application: | The mRNA Isolation Kit prepares highly purified poly (A) + RNA which may be used directly in many molecular biology applications: • RT-PCR • cDNA synthesis • Northern blotting • Northern ELISA • RNase protection assay • In vitro translation |
| Components: | • Lysis Buffer • Streptavidin-Coated Magnetic Particles • Oligo (dT)20 Probe, biotin-labeled • Washing Buffer • Water, double-distilled, PCR Grade • Storage Buffer |
| Features and Benefits: | • Save time. Isolate mRNA directly from cell lysates and tissue homogenates (isolation of total RNA not required). • Accommodate a wide range of samples. Work with both small and large scale preparations of mRNA. • Achieve highly effective purification. Generate mRNA that is intact and free of DNA and other RNAs. • Increase safety. Eliminate the use of hazardous organic solvents. |
| General description: | The mRNA Isolation Kit isolates highly purified mRNA from cells or tissue. Affinity purification is a versatile and highly specific technique for the purification of all classes of biomolecules utilizing differences in biological activities of chemical structures. The high selectivity of this technique results in good purification and high recovery. Often a concentrating effect is reached which enables large volumes to be conveniently processed. The mRNA Isolation Kit depends upon the affinity of the poly(A) + tail of mRNA for a biotin-labeled oligo (dT) probe. The probe can "pull" the mRNA selectively from a lysate without interacting with other RNA or DNA. Once formed, the biotinylated dT-A hybrids can be immobilized on solid surfaces that have been coated with streptavidin, and then washed free of unbound contaminants. |
| Other Notes: | For general laboratory use. |
| Preparation Note: | The poly (A)+ tail of mRNA hybridizes to a biotin-labeled oligo(dT) probe. Streptavidin-coated magnetic particles capture the biotinylated hybrids and the particles are separated using a magnetic particle separator. Contaminants are removed by washing, followed by elution of the mRNA from the particles using water. |
| Quality: | Tested for absence of RNases according to the current quality control procedures; function tested. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| UNSPSC | 41105500 |