Universal Protease Substrate
ROCHE/11734334001 - lyophilized, suitable for detection, pkg of 40 mg (40 μmol; 100mM)
Synonym: Substrate of protease; casein, resorufin-labeled; protease substrate, universal
Product Type: Chemical
Influence of incubation time on the casein-resorufin hydrolysis by trypsin.
Limited and complete (exhaustive) digestion of casein-resorufin by different proteases.
| application(s) | detection |
| concentration | 1 mg/mL |
| form | lyophilized |
| manufacturer/tradename | Roche |
| packaging | pkg of 40 mg (40 μmol; 100mM) |
| Quality Level | 100  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | Principle By treatment with proteases, resorufin-labeled peptides are released from resorufin-labeled casein. They cannot be precipitated by trichloroacetic acid. The concentration of these resorufin-labeled peptides in the supernatant is equivalent to the proteolytic activity present. |
| Application: | Use Universal Protease Substrate as a general substrate for proteases, and to detect trace protease activities, for example, as a contamination in enzyme preparations. It can be measured spectrophotometrically and fluorimetrically in a homogeneous assay. |
| General description: | The Universal Protease Substrate provides rapid and highly sensitive detection of trace protease activity, or determination of the effectiveness of protease inhibition. Protease activity releases resorufin-labeled peptides from the patented substrate, resorufin-labeled casein. Resorufin is N-(resorufin-4-carbonyl)- • Conveniently detect nanogram quantities of proteolytic activity in less than one hour. • Perform highly sensitive protease detection in a homogeneous assay. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Approximately 1 mg/ml Storage conditions (working solution): -15 to -25 °C An aqueous solution is stable for several months at -15 to -25° C and for 2 to 3 days at 2 to 8° C. It is recommended to store aqueous solutions in aliquots at -15 to -25° C. Repeated freezing and thawing is possible. At 15 to 25 °C the product is rapidly hydrolyzed in solution. |
| Quality: | Function test: performance-controlled in the assay for proteases. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |