Immunoprecipitation Kit (Protein A)
ROCHE/11719394001 - sufficient for 20 reactions, kit of 1, suitable for immunoprecipitation (IP)
Product Type: Chemical
| manufacturer/tradename | Roche |
| packaging | kit of 1 |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | immunoprecipitation (IP): suitable |
| usage | sufficient for 20 reactions |
| Application: | Immunoprecipitation Kit (Protein A) has been used for the immunoprecipitation of proteins from cellular extracts with Protein A Agarose. |
| General description: | Immunoprecipitation is a widely used method for the analysis of target antigens in complex mixture of proteins. The protein of interest can be concentrated and immunoaffinity -purified in one step on an analytical scale via a specific antibody. Often, immunoprecipitated proteins are functionally fully active and can be further analyzed with respect to enzymatic activity, interactions, modifications and structure. |
| General description: | Kit contains all reagents necessary for cell lysis, solubilization, stabilization, and immunopurification of proteins. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 5 components. |
| Preparation Note: | Working solution: Lysis buffer/wash buffer 1 The kit contains reagents for 125 ml of lysis buffer/wash buffer 1. Prepare at least a minimal volume of 25 ml, sufficient for four immunoprecipitations. To prepare 25 ml of lysis buffer/wash buffer 1 mix 5 ml core buffer, 3.75 ml NaCl, 2.5 ml detergent mix and 1 cOmplete tablet. Add water to a final volume of 25 ml. Solution is stable at 2 to 8 °C for 24 hours. When stored in aliquots at -15 to -25 °C, the solution is stable for at least four weeks. Mix thoroughly after thawing. Wash buffer 2 The kit contains reagents for 50 ml of wash buffer 2. 2 ml of this buffer is required for one immunoprecipitation. To prepare 50 ml of wash buffer 2 mix 10 ml core buffer, 25 ml NaCl and 0.5 ml detergent mix. Add water to a final volume of 50 ml. Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing. Wash buffer 3 The kit contains reagents for 25 ml of wash buffer 3. 1 ml of this buffer is required for one immunoprecipitation. To prepare 25 ml mix 1 ml core buffer and 0.25 ml detergent mix. Add water to a final volume of 25 ml. Solution is stable at 2 to 8 °C. For longer periods, store aliquots at -15 to -25 °C. Mix thoroughly after thawing. |
| Symbol |    GHS05,GHS07,GHS09 |
| Signal word | Danger |
| Hazard statements | H314 - H317 - H411 |
| Precautionary statements | P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P391 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |
| Components | Core Buffer; NaCl; Detergent Mix; cOmplete Protease Inhibitor Cocktail Tablets (5); Protein A Agarose ready-to-use |