PCR Buffer Set
ROCHE/11699121001 - suitable for PCR, pkg of 2 × 2 mL
Product Type: Chemical
| manufacturer/tradename | Roche |
| packaging | pkg of 2 × 2 mL |
| Quality Level | 100  |
| storage temp. | −20°C |
| technique(s) | PCR: suitable |
| Application: | For optimization of the magnesium-chloride concentration in the polymerase chain reaction (PCR) using Taq DNA Polymerase. |
| General description: | The kit-provided PCR buffer without MgCl2, in combination with the kit-provided MgCl2 stock solution, are used for the individual adjustment of the Mg2+ concentration. In most PCR applications, a concentration of 1.5 mM MgCl2 will yield satisfactory results with a dNTP concentration of 200 μM for each nucleotide. To optimize the PCR, titrate to determine the optimal Mg2+ concentration for high PCR specificity and amplicon yield. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 set containing 2 components |
| Packaging: | 1 set containing 3 components |
| Quality: | Function test: Each lot of buffer is tested in PCR. Absence of contaminants: No exo- or endonuclease activity is detectable according to the current Quality Control procedures. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 41116133 |
| Components | MgCl2 Stock Solution 25 mM; PCR Buffer without Mg2+ 10x concentrated; Alkaline Phosphatase Substrate, CSPD; Enhancer, Emerald II; Assay Buffer |