Anti-HA-Peroxidase
ROCHE/11667475001 - from mouse IgG2bκ
Synonym: antibody
Product Type: Chemical
antibody form | purified immunoglobulin |
antibody product type | primary antibodies |
assay | ≥95% (HPLC and SDS-PAGE) |
biological source | mouse |
clone | 12CA5, monoclonal |
concentration | 0.1 mg/mL |
conjugate | peroxidase conjugate |
epitope sequence | YPYDVPDYA |
form | solution |
isotype | IgG2bκ |
manufacturer/tradename | Roche |
packaging | pkg of 50 μg (500 μl) |
Quality Level | 100 |
storage temp. | −20°C |
Application: | • Use Anti-HA-Peroxidase for the immunochemical detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using: • Dot blot • Western blot Use of Anti-HA-Peroxidase eliminates the need for a secondary detection step. Note: For experiments in which sensitivity is not critical, use Anti-HA-Peroxidase (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity. |
General description: | Anti-HA-Peroxidase is a monoclonal antibody to the HA peptide, conjugated to peroxidase. Anti-HA-Peroxidase recognizes the HA nonapeptide sequence (YPYDVPDYA), derived from the human influenza virus hemagglutinin protein (amino acids 98-106). The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging". |
Immunogen: | Amino acids 98-106 from the human influenza virus hemagglutinin protein |
Other Notes: | For life science research only. Not for use in diagnostic procedures. |
Preparation Note: | Stabilizers: BSA, sucrose, 0.02% thymol Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:• Western blot: 0.1 μg/ml Working solution: 20-fold dilution of Western Blocking Reagent in 1x PBS. |
Quality: | Each lot of Anti-HA antibody is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation. |
Specificity: | Anti-HA-Peroxidase recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal). |
Specificity: | The antibody reacts with the HA epitope, a nonapeptide sequence (YPYDVPDYA) derived from the influenza hemagglutinin protein. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | does not flash |
Flash Point(C) | does not flash |
Purity | ≥95% (HPLC and SDS-PAGE) |
Storage Temp. | −20°C |
UNSPSC | 12352200 |