DIG Wash and Block Buffer Set
ROCHE/11585762001 - storage temp.:2-8°C
Synonym: wash and block buffer set, dig
Product Type: Chemical
DIG Wash and Block Buffer Set
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category | Aligned  |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| manufacturer/tradename | Roche |
| Quality Level | 100 |
| storage temp. | 2-8°C |
| Application: | The DIG Wash and Block Buffer Set is used at various steps of DIG hybridization and DIG detection. |
| General description: | Except for the Blocking solution, the 10x concentrated buffers are diluted to the desired concentrations with sterile double-distilled water (not included). Washing, blocking and detection buffers (10x concentrated) for the immunological detection of DIG - (digoxigenin-)labeled probes. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 set containing 4 components. |
| Preparation Note: | Working solution: dATP, dGTP, dTTP mixture: For one labeling reaction pipette: 1 μl dATP, (vial 2) 1 μl dGTP, (vial 4) 1 μl dTTP, (vial 5) to a reaction vial. Note: If the same type of labeled deoxyribonucleoside-triph Storage conditions (working solution): • After the first usage, the buffers should be stored further at 2 to 8 °C. We recommend to store the concentrated Blocking solution in aliquots at -15 to -25 °C. • When Autoclaved: Stock solution can be stored for several days to a week either unopened at RT or at 2 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months. |
| Quality: | The buffers are autoclaved, filtered and tested for the absence of DNases and RNases according to the current Quality Control procedures. The buffers are function tested according to the standard procedure of the DIG Nucleic Acid Detection Kit. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P264 - P280 - P302 + P352 - P332 + P313 - P337 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41105326 |
| Components | Washing Buffer 10x concentrated; Maleic acid Buffer 10x concentrated; Blocking Solution 10x concentrated; Detection Buffer 10x concentrated |