Cell Death Detection ELISA
ROCHE/11544675001 - sufficient for ≤96 tests, suitable for ELISA, kit of 1 (9 components)
Synonym: ELISA
Product Type: Chemical
| manufacturer/tradename | Roche |
| packaging | kit of 1 (9 components) |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | ELISA: suitable |
| usage | sufficient for ≤96 tests |
| Application: | Cell Death Detection ELISA has been used to measure Caspase-3 activity, intracellular reactive oxygen species (ROS) and cell apoptosis. |
| Application: | For research use only. Not for use in diagnostic procedures. Specific determination of mono- and oligonucleosomes in the cytoplasmic fraction of cell lysates. |
| General description: | The Cell Death Detection ELISA serves as photometric enzyme immunoassay for the qualitative and quantitative in vitro determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) after induced cell death. This assay is based on the quantitative sandwich-enzyme immunoassay-principle using mouse monoclonal antibodies directed against DNA and histones, respectively. The antibodies used are not species specific. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 9 components. |
| Preparation Note: | Working solution: Coating solution Predilute 1 ml Coating buffer conc. with 9 ml double dist. water. Shortly before use, dilute 1 ml anti-histone antibody (reconstituted) with 9 ml Coating buffer. Washing solution Warm the Washing buffer concentrate to 15 to 25 °C and dilute 40 ml in 360 ml double distilled water. Mix thoroughly. Sample solution Preparation of the sample solution depends on the cell system used and the extent of cell death. Example: Dilute 25 μl of sample in 225 μl Incubation buffer. Conjugate solution Dilute 1 ml Anti-DNA-POD (reconstituted) with 9 ml Incubation buffer. Substrate solution Depending on the number of samples tested, dissolve 1, 2, or 3 tablets in 5, 10, or 15 ml Substrate buffer. Allow to come to 15 to 25 °C before use. Note: The ABTS solution is light sensitive over extended periods of time. Storage conditions (working solution): Anti-histone 2 to 8 °C for 2 months Anti-DNA-POD 2 to 8 °C for 2 months Coating solution Prepare immediately before use! Washing solution 2 to 8 °C for 2 months Sample solution 2 to 8 °C for 2 months Conjugate solution Prepare immediately before use! Substrate solution 2 to 8 °C for 1 month, protected from light. |
| Reconstitution: | Anti-histone Reconstitute the lyophilizate in 1 ml double-distilled water for 10 minutes.Mix thoroughly. Anti-DNA-POD Reconstitute the lyophilizate in 1 ml double-distilled water for 10 minutes. Mix thoroughly. |
| Specificity: | Anti-histone antibody reacts with the histones H1, H2A, H2B, H3, and H4 of various species (e.g., human, mouse, rat, hamster, cow, opossum, and Xenopus). Anti-DNA-POD antibody binds to ss- and dsDNA. Therefore, the ELISA allows the detection of mono- and oligonucleosomes from various species, and may be applied to measure apoptotic cell death in many different cell systems. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H319 |
| Precautionary statements | P261 - P280 - P333 + P313 - P337 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |
| Components | Anti-histone antibody (clone H11–4); Anti-DNA-POD antibody (clone MCA-33); Coating Buffer; Washing Buffer; Incubation Buffer, ready-to-use solution; Substrate Buffer, ready-to-use solution; ABTS Substrate Tablet; Microplate Modules; Adhesive Plate Cover Foils |