RNA Molecular Weight Marker I, DIG-labeled
ROCHE/11526529910 - solution, 20 ng/μL, pkg of 200 μL (4 μg)
Product Type: Chemical
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| concentration | 20 ng/μL |
| form | solution |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry  . |
| manufacturer/tradename | Roche |
| packaging | pkg of 200 μL (4 μg) |
| Quality Level | 100 |
| storage temp. | −20°C |
| Application: | RNA Molecular Weight Marker I, DIG-labeled, is used as a size standard in northern blot analysis when the DIG System for Nucleic Acid Labeling and Detection is used. The digoxigenin-labeled RNA standard can be detected simultaneously with hybridized digoxigenin-labeled probes during the immunological digoxigenin detection reaction, allowing a length determination of the nonradioactively detected RNA on the blot. Either chemiluminescent (e.g., DIG Luminescent Detection Kit) or colorimetric (e.g., DIG Nucleic Acid Detection Kit) detection may be used. |
| Features and Benefits: | DIG RNA molecular weight markers consist of a mixture of in vitro-synthesized digoxigenin-labeled RNA chains of defined length for the molecular-weight determination of RNA species on northern blots. After gel electrophoresis of the appropriate DIG RNA molecular weight marker, under denaturing conditions, and northern transfer onto a nylon or nitrocellulose membrane, the corresponding bands are visible with the DIG immunoassay using the chromogenic substrate NBT/BCIP or the chemiluminescent substrate CSPD. |
| General description: | The fragments are prepared by in vitro transcription of linearized plasmids with SP6 or T7 RNA Polymerase in separate reactions. The transcripts are then combined at a ratio that gives bands of uniform intensity when separated by gel electrophoresis. The transcripts are labeled in a photodigoxigenin reaction so that a digoxigenin moiety is present every 200th to 300th nucleotide. This does not result in a visible gel shift. Size Range: 0.3 to 6.9 kb |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Transfer of 40 ng to 100 ng of the labeled RNA Molecular Weight Marker I per lane or 20 ng to 50 ng of RNA Molecular Weight Marker II or III per lane, depending on the reaction time in the detection step, gives a clearly visible banding pattern. Note: Taking the lane (slot / pouch) size of the agarose gel into account we recommend to load 100 ng ( for RNA marker I) and 50 ng ( for RNA marker II or III) into each slot/ pouch. |
| Sequence: | Nine fragments: 310, 438, 575, 1049, 1517, 1821, 2661, 4742, and 6948 bases. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Storage Temp. | −20°C |
| UNSPSC | 41105335 |