IsoStrip™ Mouse Monoclonal Antibody Isotyping Kit
ROCHE/11493027001 - sufficient for 10 tests
Synonym: hybridoma subtyping
Product Type: Chemical
IsoStrip Mouse Monoclonal Antibody Isotyping Kit
Test principle of the Isotype characterization with the IsoStrip Mouse Monoclonal Antibody Isotyping Kit
In the figure, mouse IgG1, kappa is shown. Cutting off the black area at the bottom of the strip is recommended for a permanent record. Procedure: 1. An antibody-containing sample is pipetted into a development tube, and agitated so that the colored latex beads are completely resuspended. 2. An isotyping strip is placed in the tube. 3. Within 1 - 5 minutes, a blue band will appear in either the kappa or lambda section of the strip, as well as in one of the class or subclass sections, indicating the class or subclass and light-chain composition of the monoclonal antibody. These blue bands will intensify as the sample moves up the strip. The positive control bands on each side of the isotyping strip should also appear, indicating the antibody-coated latex beads are functional and have traveled up the strip. After 5 minutes, the results can be interpreted.
| manufacturer/tradename | Roche |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| usage | sufficient for 10 tests |
| Application: | The kit is used for the rapid identification of class, subclass, and light-chain types of mouse monoclonal antibodies (immunoglobulins). |
| Features and Benefits: | • Reliable: Yields comparable results to the standard ELISA assay • Fast: 5 minutes from start to finish • Easy: Only 3 steps • Economical: No equipment or additional reagents required • Highly specific: No crossreactivity with FBS |
| General description: | IsoStrip Mouse Monoclonal Antibody Isotyping Kit is a simple, three-step kit for the rapid characterization of mouse monoclonal antibodies. Contents • Development Tubes, contain lyophilized latex beads, precoated with anti-mouse-Ig antibodies • Isotyping Strips, precoated with subclass- and light-chain-specific anti-mouse-Ig antibodies |
| Legal Information: | IsoStrip is a trademark of Roche |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 2 components. |
| Preparation Note: | Working solution: Dilute Sample• Remove the desired number of isotyping strips from the canister. Remove the caps from an equal number of development tubes. The tubes may be labeled with a pencil or felt-tipped lab marker for easy identification. • Dilute a sample containing the mouse monoclonal antibody in 1% BSA/ phosphate-buffered saline (PBS), pH 7.2 to 7.6. Culture supernatant samples should be diluted 1:10 to 1:100. Ascites samples should be diluted 1:20,000. These are recommended dilutions and may vary depending on the concentration of antibody in your sample. In our experience, a monoclonal antibody concentration of 0.1 to 1 g/ml of diluted sample gives the best results. 150 ml of this diluted sample will be added to the development tube. |
| Specifications: | Assay time: 5 minutes Sample material: Hybridoma supernatant, purified mouse monoclonal antibodies, ascites |
| Specificity: | Anti-mouse antibodies, bound to the latex beads, will react with any mouse monoclonal antibody regardless of its isotype.Goat anti-mouse antibodies, immobilized to the strip, specifically bind to each of the common mouse antibody isotypes (IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA), and to the light chains. Specificity for all specific antibodies beside IgML at 1.0 μg/ml: ≥ 2 IgM Lambda, at 1.0 μg/ml: ≥ 1 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 23201100 |
| Components | Development Tubes, containing lyophilized latex beads, precoated with anti-mouse-Ig antibodies; Isotyping Strips, precoated with subclass- and light-chain-specific anti-mouse-Ig antibodies |