Anti-Fluorescein-AP, Fab fragments
ROCHE/11426338910 - from sheep
Synonym: antibody
Product Type: Chemical
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | sheep |
| clone | polyclonal |
| conjugate | alkaline phosphatase conjugate |
| form | solution |
| isotype | IgG |
| manufacturer/tradename | Roche |
| packaging | pkg of 150 U (200 μl) |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | Use Anti-Fluorescein-AP, Fab fragments for the detection of fluorescein-labeled compounds using: • Dot blot • ELISA • Immunohistocytochemistr • In situ hybridization • Southern blot • Western blot • Electrochemical biosensing technique for the detection of 16S rRNA |
| Biochem/physiol Actions: | Anti-Fluorescein-AP, Fab fragments has been used in oligonucleotide capture, denaturation and detection (CDD). |
| Features and Benefits: | Contents Solution, stabilized |
| General description: | Fluorescein is synthesized from m-dialkylaminophenols or resorcinols condensed with phthalic anhydrides under harsh conditions. Fab fragments bind to G proteins. |
| General description: | This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. (provided by Ref Seq) |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | • Working concentration: The following concentrations depend on application and substrate and should be taken as a guideline. Dot blot: 150mU/ml • ELISA: 150 to 300mU/ml • Immunohistocytochemistr • In situ hybridization: 1.5 to 7.5U/ml • Southern blot: 150mU/ml • Western blot: 250 to 500 mU/ml Working solution: 100mM Tris-HCl, 150mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. |
| Specificity: | The polyclonal antibody reacts with free and bound fluorescein. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |