Anti-Fluorescein
ROCHE/11426320001 - from mouse IgG1 (clone B13-DE1)
Synonym: antibody
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | B13-DE1, monoclonal |
| conjugate | unconjugated |
| form | lyophilized |
| isotype | IgG1 |
| manufacturer/tradename | Roche |
| packaging | pkg of 100 μg |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | Use Anti-Fluorescein for the detection of fluorescein-labeled compounds using: • ELISA • Immunohistocytochemistr • In situ hybridization • Western blot Anti-Fluorescein has been used to capture amplicon in quadruple-tagging PCR and nucleic acid lateral flow assay for simultaneous detection of foodborne pathogens. |
| General description: | Monoclonal antibody to fluorescein. The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate. The antibody does not contain any protein and can hence be used for coating and labeling. Contents Lyophilizate, stabilized |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: • ELISA: 2 to 4 μg/ml • Immunohistocytochemistr • In situ hybridization: 0.2 to 0.4 μg/ml • Western blot: 0.5 to 2 μg/ml Working solution: Phosphate buffered saline, pH 7.4 Using water instead the antibody can precipitate. |
| Reconstitution: | Add 1 ml double-distilled water to a final concentration of 100 μg/ml. Reconstitute for 15 minutes. |
| Specificity: | The monoclonal antibody reacts with free and bound fluorescein. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 - H412 |
| Precautionary statements | P261 - P273 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |