dUTP
ROCHE/11420470001 - =98%, solution, 100 mM, suitable for DNA amplification
Synonym: 2′-deoxy-uridine-5′-triphosphate; deoxy-uridine-5′-triphosphate, 2′-
Product Type: Chemical
| assay | =98% |
| concentration | 100 mM |
| description | Lithium salt |
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 250 μL (25 μmol; 100 mM) |
| Quality Level | 100  |
| storage temp. | −20°C |
| technique(s) | DNA amplification: suitable |
| DNA sequencing: suitable | |
| usage | sufficient for 6,250 standard reactions (PCR) |
| Application: | DNA that contains dUTP is susceptible to hydrolysis with Uracil-DNA Glycosylase. Therefore, dUTP is most commonly used (e.g., in PCRs and RT-PCRs with Taq DNA Polymerase) to prevent carryover contamination.However, dUTP is also a substrate for other DNA synthesizing enzymes (e.g., the Kornberg DNA polymerase and the Klenow Enzyme). Therefore, dUTP can be used for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions. |
| General description: | dUTP is a 100 mM clear, colorless solution of the lithium salt (pH 7). Contents dUTP solution in water (pH 7), 100 mM, lithium salt |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Quality: | Typical analysis: • ≥98% dUTP (HPLC), =1.5% dUDP (HPLC), =0.5% dUMP (HPLC) • Absorbance determination A250/A260, A280/A260, A290/A260 |
| Unit Definition: | 1 μmol dUTP = 9.9 A260 units |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | =98% |
| Storage Temp. | −20°C |
| UNSPSC | 41116100 |