DNA Molecular Weight Marker IV
ROCHE/11418009001 - solution, pkg of 50 μg (in 200 μl)
Synonym: DNA marker
Product Type: Chemical
DNA Molecular Weight Marker IV (1% agarose gel)
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 50 μg (in 200 μl) |
| Quality Level | 100  |
| storage temp. | −20°C |
| Application: | DNA Molecular Weight Marker IV is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by: • Restriction digests • PCR • RT-PCR (Use this marker to size DNA fragments between 0.07 and 19.3 kbp). |
| General description: | Fragment mixture prepared by cleavage of equimolar amounts of λDNA and pSPTBM20 DNA with restriction endonucleases Sty I and Sau I. Ready-to-use solution, 250 μg/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). Size Range: 0.07 to 19.3 kbp |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Storage conditions (working solution): 2 to 8 °C Once thawed, store at 2 to 8 °C. |
| Sequence: | The mixture contains 14 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 74, 421, 697, 925, 1150, 1489, 1882, 2322, 2690, 3140, 4254, 5526, 7743, and 19329 as determined by computer analysis of λDNA and pSPTBM 20 sequences. Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 41105335 |