Insulin, human
ROCHE/11376497001 - recombinant (yeast)
Synonym: Insulin human
CAS Number: 11061-68-0
Empirical Formula (Hill Notation): C257H383N65O77S6
Molecular Weight: 5807.57
MDL Number: MFCD00131380
Linear Formula: C257H383N65O77S6
Product Type: Chemical
| assay | >98% (SDS-PAGE) |
| form | lyophilized (clear, colorless solution after reconstitution) |
| impurities | <0.1 EU/μg endotoxin (LAL test) |
| InChI key | PBGKTOXHQIOBKM-FHFVDXKLSA |
| manufacturer/tradename | Roche |
| mol wt | 5,700 Da |
| packaging | pkg of 100 mg |
| Quality Level | 100  |
| solubility | water: soluble |
| specific activity | >26 units/mg protein (At least the same specific activity (EC<sub>50</sub>) compared to the indicated standard is guaranteed.) |
| sterility | non-sterile; 0.2 μm filtered |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01308  |
| Application: | Insulin shows a broad range of activities on a variety of somatic cells. Recombinant human insulin can be used to stimulate growth and proliferation of cultured cells and to investigate insulin activity on sensitive cells used in research studies. It is also a component of serum-free media formulations for most primary cells and cell lines. |
| Biochem/physiol Actions: | Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat. |
| General description: | Recombinant Insulin, human, is produced in yeast and purified by standard chromatographic techniques. Insulin regulates blood glucose. In muscles and adipocytes it stimulates glucose intake and metabolism. It also controls the expression and various enzymes. |
| Other Notes: | InChI: 1S/C257H383N65O77S6/c1-29 |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Lyophilizate from a hydrochloric acid solution (pH 2.3) (crystalline insulin) |
| Preparation Note: | Working concentration: 1-10 μg/ml Recommended concentration for serum-free cell culture is 1-10 μg/ml. The concentration of insulin required for stimulation of cell growth in almost all cases is extraordinarily high compared with the physiological concentration. Insulin may be mimicking insulin-like growth factors (IGFs, somatomedins) for some cell lines, and high insulin concentrations may be necessary to occupy receptors which have a high affinity for IGFs and a lower affinity for insulin. Working solution: Dissolve Insulin, human, recombinant (100 mg or 500 mg), in sterile, double-dist. water (final concentration: 10 mg/ml). Storage conditions (working solution): -15 to -25 °C The reconstituted, undiluted solution is stable at -15 to -25 °C. Note: Avoid repeated freezing and thawing. |
| Quality: | Endotoxin level: <0.1EU/μg (LAL-test) Note: 1 EU corresponds to 0.1ng |
| Reconstitution: | Reconstitute in sterile double-distilled water (final concentration 10 mg/ml), further dilution with PBS (phosphate buffered saline) or medium containing 1 mg/ml (0.1%) BSA (bovine serum albumin) [or HSA (human serum albumin)], or 1 to 10% serum. |
| Sequence: | A-chain: 21 AS, B-chain: 30 AS, connected by two disulfide bridges |
| Sequence: | Two polypeptide chains (A-chain: 21 amino acids, B-chain: 30 amino acids, connected by two disulfide bridges) identical to natural, human insulin. |
| Specificity: | Insulin, human, is active on most mammalian cells. |
| Unit Definition: | EC50 definition: The concentration of human insulin that is required to support half-maximal stimulation of cell proliferation (MTT cleavage) with 3T3 (A31) cells. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >98% (SDS-PAGE) |
| activity | specific activity: >26 units/mg protein (At least the same specific activity (EC<sub>50</sub>) compared to the indicated standard is guaranteed.) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |