Endoproteinase Arg-C Sequencing Grade
ROCHE/11370529001 - from Clostridium histolyticum
Synonym: protease
Product Type: Chemical
| biological source | Clostridium histolyticum |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | Mr 59 kDa |
| optimum pH | 7.2-8.0 |
| packaging | pkg of 3 × 5 μg |
| parameter | 37 °C optimum reaction temp. |
| storage temp. | 2-8°C |
| Application: | Use Endoproteinase Arg-C Sequencing Grade for the specific cleavage of proteins and peptides for peptide mapping, fingerprinting, and sequence analysis. The protease is suitable to digest proteins in solution, in gels, or on blotting membranes. |
| General description: | Endoproteinase Arg-C Sequencing Grade is isolated from Clostridium histolyticum as a highly purified and specific cysteine protease. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and β-mercaptoethanol. Inhibitors: Oxidizing agents, sulfhydryl reactants (e.g., TLCK), EDTA, Co2+, Cu2+, Cd2+. Citrate, borate, and Tris anions partially inhibit. Optimum pH: 7.2 - 8.0 Storage conditions (working solution): -15 to -25°C For long-term use, the solution should be gently flushed with a stream of nitrogen and stored at -15 to -25°C. The reconstituted incubation buffer is stable for several months at -15 to -25°C. |
| Quality: | Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC. |
| Reconstitution: | The lyophilized endoproteinase Arg-C sequencing grade is reconstituted in 50 μl double-dist. water to give a final concentration of 50 mM Tris-HCl buffer, 10 mM CaCl2, 5 mM EDTA, pH 8.0. The lyophilized activation solution is dissolved in 100 μl double-dist. water resulting in a final 50 mM dithiothreitol-(DTT-)conc |
| Specificity: | Endoproteinase Arg-C Sequencing Grade is a cysteine protease that specifically cleaves peptide, ester, and amide bonds at the C-terminal side of arginine residues. The specificity is primarily to arginine residues, although hydrolysis proceeds to a minor degree in most lysine-containing substrates. Reducing agents (such as DTT) and Ca2+ are required for full activity. The specificity of Endoproteinase Arg-C Sequencing Grade is tested with the oxidized B-chain of insulin (insulin Box) as a substrate. High concentrations of Endoproteinase Arg-C Sequencing Grade are incubated for prolonged incubation times to detect traces of impurities such as contaminating proteases. Recognition sites: C-terminal side of arginine residues, other recognition sites with lower efficiency. |
| Symbol |    GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 - H334 |
| Precautionary statements | P261 - P264 - P280 - P304 + P340 - P305 + P351 + P338 + P310 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.4.22.8  ( BRENDA  | IUBMB ) |
| UNSPSC | 23201100 |