Nutridoma™-CS
ROCHE/11363743001 - suitable for hybridoma, solution, pkg of 10 mL (50x)
Synonym: serum replacements
Product Type: Chemical
| concentration | 50 × |
| form | solution |
| impurities | mycoplasma, tested |
| 100 EU/mL endotoxin (LAL test) | |
| manufacturer/tradename | Roche |
| packaging | pkg of 10 mL (50x) |
| Quality Level | 100  |
| solubility | water: miscible |
| sterility | sterile; 0.2 μm filtered |
| storage temp. | −20°C |
| technique(s) | cell culture | hybridoma: suitable |
| Application: | Contents Solution, 50× concentrated, Biochemically defined, filtered through 0.2μm pore size membrane, serum-free medium supplement composed of albumin, insulin, transferrin, several cytokines, a cholesterol source, and other defined organic and inorganic compounds. Nutridoma-CS contains human proteins. |
| Application: | Biochemically defined supplement for the replacement of fetal bovine serum (FBS) in cultures of hybridoma cells. Nutridoma-CS supports the growth of SP2/0-, P3x63Ag8.653-, and NS-1-derived hybridomas. It is specifically formulated to optimize the growth of freshly fused hybridomas during selection and cloning procedures in serum-free cell culture without feeder cells. |
| Application: | Nutridoma™-CS has been used as a supplement in neurobasal medium for culturing human embryonic stem cell (hESC) lines. |
| General description: | Nutridoma-CS is a serum replacement solution which helps in cell proliferation when added to a suspension. Its major component is β-D xylopyranose which has ring shaped sugar allowing the cells to grow in a suspension even without serum. |
| Legal Information: | Nutridoma is a trademark of Roche |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution (50x concentrated, pH 7.4); sterile. The protein concentration is less than 1 mg/ml for 2% (1x) working concentration. |
| Preparation Note: | Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and β-mercaptoethanol. |
| Unit Definition: | Endotoxin (LAL): 100 EU/ml, mycoplasma tested |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |