Hybridoma Fusion and Cloning Supplement
ROCHE/11363735001 - solution, suitable for hybridoma, Mycoplasma, tested
Synonym: HFCS
Product Type: Chemical
Hybridoma Fusion and Cloning Supplement
| description | HFCS |
| form | solution |
| impurities | Mycoplasma, tested |
| 10 EU/mL Endotoxin (LAL) | |
| manufacturer/tradename | Roche |
| packaging | pkg of 10 mL (50x) |
| Quality Level | 100  |
| solubility | water: miscible |
| sterility | 0.2 μm filtered |
| storage temp. | −20°C |
| technique(s) | cell culture | hybridoma: suitable |
| Analysis Note: | Biological activity: Assayed for high cloning efficiency of a hybridoma cell line. |
| Application: | Hybridoma Fusion and Cloning Supplement (HFCS) is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. HFCS replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen. |
| General description: | Medium supplement composed of albumin, insulin, transferrin, cytokines, a cholesterol source, other defined organic and inorganic compounds and fetal calf serum (final concentration: 0.5%). HFCS contains human proteins. The raw material from which the human proteins were isolated, has been tested for the presence of Hepatitis B surface Antigen (HBsAg) and HIV-1/2 antibodies and found to be negative (according to current quality control procedures). |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution (50x concentrated, pH 7.4); filtered through 0.2 μm pore size membrane |
| Preparation Note: | Working concentration: HFCS (50x) is diluted 1:50 (v/v) with basal medium. It is strongly suggested that you use RPMI 1640. The final medium should also contain L-glutamine and sodium bicarbonate. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |