Anti-Digoxigenin
ROCHE/11333089001 - from sheep
Synonym: anti-digoxigenin; digoxigenin
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | sheep |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized |
| isotype | IgG |
| manufacturer/tradename | Roche |
| packaging | pkg of 200 μg |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Analysis Note: | No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone. |
| Application: | Anti-Digoxigenin has been used in DNA tethering. It has been used to attach DNA molecule to the glass surface of the flow cell. |
| Application: | Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using: • ELISA • Immunohistocytochemistr • In situ hybridization • Western blot |
| Biochem/physiol Actions: | In the presence of Na+, Mg2+ and ATP, digoxigenin inhibits sodium pumps. |
| General description: | Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems. |
| General description: | Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes. It allows faster detection, is less hazardous and has an increased shelf life. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline: • ELISA: for coating: 2 to 4 μg/ml • Immunohistocytochemistr • In situ hybridization: 0.2 to 0.4 μg/ml • Western blot: 0.5 to 2 μg/ml Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4 |
| Preparation Note: | After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. |
| Reconstitution: | Add 1 ml PBS to a final concentration of 200 μg/ml. |
| Specificity: | The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |