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Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester

ROCHE/11333054001 - >90% (elementary analysis), pkg of 5 mg, powder, pH range 2-13

Synonym: Digoxigenin NHS-ester; dig-nhs-ester; ε-(Digoxigenin-3-0-acetamido)caproic acid N-hydroxysuccinimide ester; DIG NHS

CAS Number: 129273-26-3
Empirical Formula (Hill Notation): C35H50N2O10
Molecular Weight: 658.78
MDL Number: MFCD02093394
Linear Formula: C35H50N2O10
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-11333054001 5 mg
 $253.00
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assay >90% (elementary analysis)
form powder
InChI 1S/C35H50N2O10/c1-33-13-11-23(45-20-28(39)36-15-5-3-4-6-31(42)47-37-29(40)9-10-30(37)41)17-22(33)7-8-25-26(33)18-27(38)34(2)24(12-14-35(25,34)44)21-16-32(43)46-19-21/h16,22-27,38,44H,3-15,17-20H2,1-2H3,(H,36,39)/t22-,23+,24-,25-,26+,27-,33+,34+,35+/m1/s1
InChI key KHNDABJZSPPYLE-FUGFVFQCSA-N
manufacturer/tradename Roche
mol wt Mr 658.8
packaging pkg of 5 mg
pH range 2-13
Quality Level 100 
SMILES string [H][C@]12CC[C@]3([H])[C@]([H])(C[C@@H](O)[C@]4(C)[C@H](CC[C@]34O)C5=CC(=O)OC5)[C@@]1(C)CC[C@@H](C2)OCC(=O)NCCCCCC(=O)ON6C(=O)CCC6=O
solubility DMF: soluble
  DMSO: soluble
  ethanol: soluble
storage temp. −20°C
Application: Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester has been used to label the sequence (III) for the linker molecule sequence (VIII).
Biochem/physiol Actions: Digoxigenin, a non-radioactive immune-tag is mainly used for labeling proteins and in situ hybridization.
General description: Chemical reagent for the labeling of proteins and oligonucleotides. One reactive group is able to interact with NH2-groups (of proteins or oligonucleotides), another reactive group (Digoxigenin) reacts with Anti-Digoxigenin and thus can be detected.
Other Notes: For life science research only. Not for use in diagnostic procedures.
Preparation Note: Working concentration: 1 to 5 mM
Working solution: Preparation of working solution

Under stirring 0.327 mg digoxigenin-3-O-methylcarbonyl- ε-aminocaproic acid -N-hydroxysuccinimide ester is dissolved in 8.18 μl DMSO or ethanol.
Principle: The activated ester reacts with amino groups under mild conditions. The digoxigenin moiety can be introduced into proteins or 5′-amino substituted oligonucleotides.
Purity >90% (elementary analysis)
Storage Temp. −20°C
UNSPSC 41105600

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