5-Bromo-2′-deoxy-uridine Labeling and Detection Kit II
ROCHE/11299964001 - sufficient for ≤100 tests, storage temp.:-20°C
Synonym: 5-BrdU; 5-
Product Type: Chemical
Principle of the BrdU Labeling and Detection Kit II (AP).
| manufacturer/tradename | Roche |
| Quality Level | 100  |
| storage temp. | −20°C |
| usage | sufficient for ≤100 tests |
| Application: | 5-Bromo-2′-deoxy-uridine Labeling and Detection Kit II has been used in: • labeling of tooth roots for histology • immunostaining of mice frontal sections • immunofluorescence imaging of hepatocellular carcinoma sections |
| Application: | The kit is used for the detection of BrdU incorporated into cellular DNA by immunohistocytochemistry. |
| Features and Benefits: | • Safe: No radioisotopes are used. • Easy to perform: Follows a standard immunohistochemistry protocol. • Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU. • Flexible: Allows double-labeling protocols. |
| General description: | Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [ 3H]-thymidine, into cellular DNA, followed by autoradiography. Alternatively, 5-bromo-2′-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody. |
| General description: | Immunohistocytochemical assay for the detection of 5-bromo-2′-deoxy-uridine (BrdU) incorporated into cellular DNA. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Packaging: | 1 kit containing 7 components. |
| Preparation Note: | Working concentration: Working concentration of the labeling reagent corresponds to the WC of the In Situ Cell Proliferation Kits. Sample material: Cell culture: adherent cells, suspension cells, organ or explant cultures. Frozen or paraffin-embedded tissue sections (after in vivo labeling). |
| Principle: | Samples prelabeled with BrdU are fixed with ethanol, then incubated with a monoclonal antibody to BrdU, which contains an optimized mixture of nucleases. These nucleases generate single-stranded DNA fragments that allow binding of the antibody to BrdU. Next, an alkaline phosphatase (AP)-labeled antibody to mouse immunoglobulin is added, then bound to the anti-BrdU antibody. The sample is then incubated with the AP substrate and NBT/ BCIP, which is metabolized to form a colored reaction product. The sample is evaluated using a phase-contrast microscope. |
| Specificity: | Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2′-deoxy-uridine, and shows cross-reactivity with 5-iodo-2′-deoxy-uridine (10%). Anti-BrdU shows no cross-reactivity with 5-fluoro-2′-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine. |
| Symbol |    GHS02,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H226 - H312 + H332 - H317 - H319 - H360D |
| Precautionary statements | P201 - P210 - P261 - P280 - P308 + P313 - P370 + P378 |
| WGK Germany | WGK 2 |
| Flash Point(F) | 136.4 °F |
| Flash Point(C) | 58 °C |
| Storage Temp. | −20°C |
| UNSPSC | 41116133 |
| Components | BrdU Labeling Reagent 1,000x concentrated; Washing Buffer concentrate 10x concentrated; Incubation Buffer; Anti-BrdU antibody, contains nucleases for DNA denaturation; Anti-mouse Ig-alkaline Phosphatase antibody; NBT; BCIP |