Interferon-γ, mouse (mIFN-γ)
ROCHE/11276905001 - recombinant (E. coli)
Synonym: mIFN
Product Type: Chemical
| assay | >95% (SDS-PAGE) |
| biological source | mouse |
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 100,000 U (20 μg, 1 ml) |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| specific activity | >5*10^6 U/mg ((mIFN- γ, NIH, reference standard, Gg 02–901-533, at least the same specific activity (EC 50 ) compared to the indicated standard is guaranteed.) |
| storage temp. | −20°C |
| Application: | Interferon-γ, mouse (mIFN-γ) has been used in cell culture. |
| Application: | Recombinant mouse interferon-γ is a valuable tool for the study of IFN-γ (interferon-γ) actions in mouse model systems. |
| Biochem/physiol Actions: | Interferon-γ (IFNγ) participates in cell-mediated immunity and has pro-inflammatory effects on immunocytes and target tissue. Its functions vary and is tissue specific. IFNγ induces T-cell-mediated immune response by increasing MHC-I expression on target cells and acts through IFNγ receptor, which is expressed on both immune and non-immune cells. IFNγ is associated with the development of colitis and Sjögren Syndrome. |
| General description: | IFN-γ (interferon-γ) is produced by natural killer T cells, macrophages, Th1 CD4 T cells and activated CD8 T cells and the production increases with age. |
| General description: | Recombinant, Interferon-γ, mouse (mlFN-γ) is produced in E. coli and purified by standard chromatographic techniques. Primary structure: One polypeptide chain (131 amino acids) identical to mouse, natural IFN- ? (133 amino acids), but lacking the last two amino acids at the carboxy-terminus and not glycosylated. Glycosylation is not essential for biological activity. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng. |
| Physical form: | Solution, filtered through 0.2 μm pore size membrane, 100 000 U/ml (20 μg/ml) in PBS (phosphate buffered saline) and BSA (bovine serum albumin),1 mg/ml [purity of BSA: 98%, endotoxin (LAL): < 1 EU/mg BSA]. |
| Preparation Note: | Working solution: Dilute the concentrated mlFN-γ solution (100,000 U/ml) with PBS or culture medium containing BSA, 1 mg/ml (0.1%) or serum, 1 to 10%. Storage conditions (working solution): -15 to -25 °C Store the solution in aliquots at -15 to -25 °C. Avoid repeated freezing and thawing. |
| Quality: | • Purity: >95% (SDS-PAGE) • Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test) |
| Sequence: | Chain Length 131 AA |
| Sequence: | The primary structure of recombinant Interferon-γ, mouse (mIFN-γ) is identical to that of natural, mouse IFN-γ (one polypeptide chain, 131 amino acids), however, recombinant mIFN-γ lacks the last two amino acids at the C-terminus and is not glycosylated. Glycosylation is not essential for biological activity. |
| Specificity: | Species specificity: mouse |
| Unit Definition: | EC 50 definition : The amount of mIFN- γ that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN-γ reference standard (Gg 02–901-533) (L cells-EMC virus/cytopathic effect) (1 unit equals =0.2 ng/ml). |
| Unit Definition: | EC50 definition: One unit is defined as the amount of mlFN-γ that is required to produce equivalent antiviral activity to that expressed by 1 unit of the NIH IFN-γ reference standard (Gg 02-901-533). (L cells-EMC virus/cytopathic effect) (1 unit equals ≤0.2 ng/ml). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | >95% (SDS-PAGE) |
| activity | specific activity: >5*10^6 U/mg ((mIFN- γ, NIH, reference standard, Gg 02–901-533, at least the same specific activity (EC 50 ) compared to the indicated standard is guaranteed.) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |