Quick Spin Columns for radiolabeled RNA purification
ROCHE/11273990001 - Sephadex G-25, fine
Synonym: RNA purification; spin column
Product Type: Chemical
Principle
| manufacturer/tradename | Roche |
| packaging | pkg of 20 columns |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| Application: | Quick Spin Columns for radiolabeled RNA purification are ready-to-use disposable column units designed to quickly and efficiently separate very small (>12 bases) unlabeled or radioactively labeled RNA from nucleotides and other small molecules (e.g., after a labeling reaction). Quick Spin columns are designed for use in low-speed, swinging-bucket centrifuges. Remove unincorporated precursors from DNA labeled by nick translation, end labeling, polymerization reactions, and other labeling techniques. Use the highly purified radiolabeled DNA in Southern blotting and other downstream applications. |
| Features and Benefits: | • Save time and effort with ready-to-use columns. • Increase yields of nucleic acid in less time and with fewer steps. • Ensure optimal performance and reproducibility with quality tested columns: High recovery of radiolabeled RNA Maximum retention of unincorporated nucleotides Absence of RNases |
| General description: | Quick Spin Columns are produced with material shown to have a ≥80% RNA recovery. All the tedious, time-consuming steps involved in preparing columns have already been performed. Quick Spin Columns are pre-packed, pre-swollen, and quality tested to ensure maximal retention of unincorporated nucleotides (≥95%) and absence of DNase and RNase contamination according to the current Quality Control procedures. Contents: Pre-packed, pre-swollen, pre-spun columns. Suspension of Sephadex G-25 in STE buffer (10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM NaCl). |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Principle: | Quick Spin columns contain gel filtration matrices which allow large molecules (e.g., DNA or RNA) to pass through quickly while retaining small molecules (e.g., nucleotides). The Quick Spin format improves the molecular sieving concept by using centrifugation to separate DNA or RNA rapidly and cleanly from small contaminants. |
| Quality: | RNases are not detected , according to the current Quality Control procedures. |
| Specifications: | Recovery of labeled RNA: >80% (poly[A], approximately 500 bases) Retention of unincorporated mononucleotides: >95% Exclusion limit for RNA: <12 bases Sample size: up to 50 μl Time required: 10 minutes |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Storage Temp. | 2-8°C |
| UNSPSC | 41115700 |