Interleukin-2, mouse (mIL-2)
ROCHE/11271164001 - recombinant (E. coli)
Synonym: mIL-2; mouse interleukin-2
Product Type: Chemical
| assay | >95% (SDS-PAGE) |
| biological source | mouse |
| form | solution |
| impurities | <0.1 EU/μgtested (LAL test) |
| manufacturer/tradename | Roche |
| mol wt | 17,200 Da |
| packaging | pkg of 10,000 U (5 μg, 1 ml) |
| potency | <0.5 ng/mL EC50 |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | inhibition assay: suitable |
| UniProt accession no. | P04351  |
| Application: | Recombinant murine IL-2 is produced in E. coli and exhibits the following: • Supports the growth of murine CTLL cells (murine T cell line), but not that of human T-cells. • Strongly inhibits the binding of recombinant human IL-2 to murine responder cells. • Weakly inhibits the binding to human responder cells. • Shares identical biological and immunological activities with human IL-2. • Is a convenient tool for extensive studies of the pharmacological and physiological activities of IL-2 in murine models. • used for the stimulation of murine T-cells |
| General description: | Contents: 10,000 U/ml solution in PBS and 1 mg/ml BSA, filtered through a 0.2 μm pore size membrane |
| General description: | Interleukin-2 (IL-2, also known as T-cell growth factor, TCGF) is a lymphokine produced by lectin- or antigen-activated T-cells. It plays an important immunoregulatory role. This factor was first identified by its ability to promote the long-term in vitro proliferation of activated T-cells. It also promotes the generation and proliferation of cytotoxic T-cells, natural killer (NK) cells, and lymphokine-activated killer (LAK) cells. IL-2 also induces other lymphokines such as interferon-γ and B-cell growth factor (BCGF-1). |
| General description: | Recombinant Interleukin-2, mouse (mIL-2), is produced in E. coli and purified by standard chromatographic methods. |
| Other Notes: | Specific activity/EC 50: >2 x 106 U/mg, <0.5 ng/ml (hIL-2, NIBSC, 1st international standard, 86/504), at least the same specific activity (EC50) compared to the indicated standard is guaranteed. Note: The biological activity of this product may vary in different in vitro applications. Determine the optimal concentration range for specific applications. EC 50 definition/Unit definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals =0.5 ng). |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working solution: Dilute the concentrated IL-2 solution (10,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum alburnin)], or 1 to 10% serum (v/v). Storage conditions (working solution): -15 to -25 °C Note: Avoid repeated freezing and thawing. |
| Quality: | Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test) |
| Sequence: | Chain Length 149 AA |
| Sequence: | One polypeptide chain (149 amino acids), identical to natural mouse IL-2, but not glycosylated. Glycosylation is not essential for biological activity. |
| Specificity: | Mouse IL-2 is effective on mouse cells, but not on human cells. |
| Unit Definition: | EC50 definition: The amount of mIL-2 that is required to support half-maximal stimulation of cell proliferation (XTT cleavage) with CTLL-2 cells (1 unit equals ≤0.5 ng). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |