Phytohemagglutinin-L (PHA-L)
ROCHE/11249738001 - from Phaseolus vulgaris
Synonym: PHA-L
Product Type: Chemical
| application(s) | hematology |
| assay | >95% (SDS-PAGE) |
| form | lyophilized (clear, colorless solution after reconstitution) |
| manufacturer/tradename | Roche |
| packaging | pkg of 5 mg |
| Quality Level | 100  |
| sterility | non-sterile; 0.2 μm filtered |
| storage temp. | 2-8°C |
| Application: | Phytohemagglutinin-L (PHA-L) consists of only L-type subunits (isolectin L4, "leuko-agglutinin"), and is well suited for high-efficiency induction and the functional analysis of T-lymphocytes. It is highly purified and tested for high-efficiency stimulation of human lymphocytes. It is also used as positive controls for peripheral blood mononuclear cells (PBMC) stimulation. |
| Biochem/physiol Actions: | Phytohemagglutinin (PHA) has mitogenic and potent cell agglutinating activities. It binds to T-cell membranes and stimulates cell division and metabolic activity. PHA has the ability to stimulate close contacts between cell membranes. |
| General description: | PHA-L is purified from Phaseolus vulgaris (red kidney bean) by standard chromatographic techniques. Phytohemagglutinin (PHA), the lectin extract from the red kidney bean, consists of only L-type subunits (isolectin L4, "leuko-agglutinin"). It is an N-acetylgalactosamine/gala |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Preparation Note: | Working concentration: 1 to 5 μg/ml Approximately 1 to 5 μg/ml for the stimulation of human peripheral blood lymphocytes Storage conditions (working solution): -15 to -25 °C The reconstituted solution is stable at -15 to -25 °C. Note: Store the reconstituted solution in aliquots at -15 to -25 °C. Avoid repeated freezing and thawing. |
| Sequence: | PHA-L is a tetramer of 4 L-type subunits held together by noncovalent forces. |
| Specifications: | Biological activity: <5μg/ml for maximal stimulation of DNA synthesis (BrdU incorporation) with human peripheral blood lymphocytes |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 23201100 |