Laminin
ROCHE/11243217001 - from mouse Engelbreth-Holm-Swarm (EHS) sarcoma
Synonym: Laminin from Engelbreth-
CAS Number: 114956-81-9
MDL Number: MFCD00081739
Product Type: Chemical
| assay | ≥90% (SDS-PAGE) |
| biological source | mouse (Engelbreth-Holm-Swarm (EHS) sarcoma) |
| form | solution |
| manufacturer/tradename | Roche |
| mol wt | 900 kDa |
| packaging | pkg of 1 mg (2 ml) |
| Quality Level | 100  |
| sterility | sterile; sterile-filtered |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | Q61292  |
| Application: | Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives. |
| Application: | Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives. |
| General description: | Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. Biological activity: Tested for the promotion of adherence of HT-1080 cells. Species specificity: Active on most mammalian cells. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile |
| Physical form: | Solution, filtered through 0.2 ?m pore size membrane |
| Preparation Note: | Working concentration: 2 to 5 μg/cm2 2 to 5 μg/cm2 for the coating of cell culture vessels Storage conditions (working solution): 2 to 8 °C The undiluted solution is stable at 2 to 8 °C for at least 3 months. The solution should be thawed carefully at 2 to 8 °C or on ice. |
| Quality: | Purity: ≥90% (SDS-PAGE) (as laminin nidogen-complex, 1:1) |
| Sequence: | Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |