Anti-Bromodeoxyuridine
ROCHE/11170376001 - from mouse IgG1 (clone: BMC9318)
Synonym: anti-BrdU; antibody
Product Type: Chemical
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| assay | 90% (HPLC and SDS-PAGE) |
| biological source | mouse |
| clone | BMC9318, monoclonal |
| conjugate | unconjugated |
| form | solution |
| isotype | IgG1 |
| manufacturer/tradename | Roche |
| packaging | pkg of 50 μg (500 μl) |
| Quality Level | 100  |
| storage temp. | −20°C |
| Analysis Note: | Anti-Bromodeoxyuridine shows 10% cross reaction with iodo-deoxyuridine, but no cross reaction to fluoro-deoxyuridine. No cross reaction to any endogenous thymidine or uridine. Cross reactivity with 5-Br-UTP has not been tested but it is suggested that there is a good chance for reaction, because the only difference is an absent hydroxyl group on the ribose distal to the bromine substitution. |
| Application: | Anti-bromodeoxyuridine (Anti-BrdU) antibody is suitable for monitoring proliferating cells in blood, tissues, and tumors, as well as for determining BrdU incorporation on a single-cell level using: • Flow cytometry • Immunohistocytochemistr • Cryosections • Paraffin sections |
| General description: | Anti-Bromodeoxyuridine is a monoclonal antibody to 5-bromo-2′-deoxyuridine-5 |
| Immunogen: | The epitope is apparently inside the DNA helix. DNA has to be denatured to ssDNA before antibody efficiently binds to DNA-BrdU. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution, stabilized with phosphate buffered saline, pH 7.4, containing 0.09% (w/v) sodium azide and 0.2% (w/v) gelatin. |
| Preparation Note: | Working concentration: Flow cytometry: 2 μg/ml (0.2 μg/100 μl/106 cells); Immunohistocytochemistry: 6 μg/ml Working concentration of conjugate depends on application and substrate. Dilutions should be made in PBS (pH 7.4) containing 0.1% BSA to maintain stability of the antibody. |
| Quality: | The antibody is ≥90% pure as determined by SDS-PAGE with Coomassie-blue staining, and by HPLC. |
| Specifications: | Preparation: BALB/c mice were immunized with a bromodeoxyuridine-bovine serum albumin conjugate. Lymphocytes isolated from the spleen were fused with Ag8.653 myeloma cells to create the BMC 9318 clone. The antibody was produced in ascites in BALB/c mice and purified by ion-exchange chromatography. No. of tests: 250 (Flow cytometry) |
| Specificity: | The antibody specifically binds to bromodeoxyuridine and crossreacts with iodouridine (10%). Anti-bromo-deoxyuridine does not crossreact with fluorodeoxy-uridine, nor with any endogenous cellular components such as thymidine or uridine. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Purity | 90% (HPLC and SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |