Interleukin-6, human (hIL-6)
ROCHE/11138600001 - recombinant (E. coli)
Synonym: hIL-6; interleukin-6 human
Product Type: Chemical
| assay | >98% (SDS-PAGE) |
| biological source | human |
| form | solution |
| impurities | <0.1 EU/μgtested (LAL test) |
| manufacturer/tradename | Roche |
| mol wt | 20,600 Da |
| packaging | pkg of 200,000 U (2 μg, 1 ml) |
| potency | <0.01 ng/mL EC50 |
| Quality Level | 100  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | hybridoma: suitable |
| UniProt accession no. | P05231  |
| Analysis Note: | Specific activity/EC50: >1 x 108 U/mg, <0.01 ng/ml (hIL-6, NIBSC, interim standard, 88/514), at least the same specific activity (EC50) compared to the indicated standard is guaranteed. |
| Application: | Recombinant IL-6, human, is a growth factor for murine and human B-cells. It can be used to replace feeder cells in the preparation of murine and human hybridomas. |
| Biochem/physiol Actions: | Interleukin-6 participates in several pleiotropic functions in various types of cells through its specific receptor (IL-6-R). It has been suggested that interleukin-6 may participate in the interaction between neuroendocrine and immune system by activating adrenocorticotropic hormone secretion. It has also been reported that IL-6 stimulates the bonding of receptor (IL-6-R) with a non-ligand-binding membrane glycoprotein, gp130. |
| General description: | Interleukin-6, human (hIL-6), is produced in E. coli and purified by standard chromatographic techniques. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution, filtered through 0.2 μm pore size membrane. |
| Preparation Note: | Working concentration: 10 to 100 U/ml (0.1 1 ng/ml) 10 to 100 U/ml (0.1 to 1 ng/ml) A concentration of 100 U/ml (1 ng/ml) is recommended for the preparation of B-cell hybridomas Working solution: Dilute the concentrated IL-6 solution (200,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum albumin)] or 1 to 10% serum. Storage conditions (working solution): -15 to -25 °C Store the solution in aliquots at -15 to -25 °C. Note: Avoid repeated freezing and thawing. |
| Quality: | Purity: 95% pure as determined by HPLC or SDS-PAGE [Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test). Note: 1 EU corresponds to 0.1 ng |
| Reconstitution: | Dilute the concentrated IL-6 solution (200,000 U/ml) with PBS or culture medium containing 1 mg/ml BSA [or HSA (human serum albumin)] or 1 to 10% serum. |
| Sequence: | Chain Length 184 AA |
| Sequence: | The primary structure of recombinant human IL-6 is identical to that of natural human IL-6 (one polypeptide chain, 184 amino acids), however, it contains an altered sequence within the first 15 amino-terminal amino acids. |
| Specificity: | Recombinant IL-6, human is effective on mouse and human cells. IL-6, a 26 kD protein, also known as B-cell stimulatory factor 2 (BSF-2, BCDF), interferon-β2 (IFN-β2), hybridoma/plasmacytoma growth factor (H(P)GF), and hepatocyte-stimulating factor is produced by many cell types (e.g., T-cells, monocytes, fibroblasts, endothelial cells). IL-6 induces the terminal maturation of activated B-cells into antibody-producing cells (B-cell differentiation activity. The hepatic acute phase response is induced by IL-6 (hepatocyte-stimulating activity). It was shown that IL-6 and IL-3 act synergistically to support the proliferation of hematopoietic stem cells (hemapoietic activity), however, IL-6 does not have antiviral activity, although it has been called IFN-β2. High affinity receptors for IL-6 are found on many cell types. |
| Unit Definition: | EC50 definition: The amount of hIL-6 that is required to support half-maximal stimulation of cell proliferation (MTT cleavage) with 7TD1 cells (1 unit equals <0.01 ng). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| Purity | >98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |