Erythropoietin, human (hEPO)
ROCHE/11120166001 - recombinant (CHO cells)
Synonym: EPO, human
Product Type: Chemical
| application(s) | hematology |
| assay | >98% (HPLC and SDS-PAGE) |
| biological source | human |
| form | solution |
| impurities | <1 EU/μgtested (LAL test) |
| manufacturer/tradename | Roche |
| packaging | pkg of 250 U (2.5 μg, 1 ml) |
| Quality Level | 100  |
| recombinant | expressed in CHO cells Hamster |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | P01588  |
| Application: | Erythropoietin (EPO) is a glycoprotein which stimulates proliferation and differentiation of erythroid precursor cells (CFU-E, BFU-E) to more mature erythrocytes. |
| Biochem/physiol Actions: | Erythropoietin (EPO) controls the synthesis of RBCs (red blood cells) by inducing the proliferation and differentiation of erythroid precursor cells. As it induces erythropoiesis, EPO can be used to treat anemia of cancer patients. |
| General description: | Recombinant, Erythropoietin, human (hEPO), is produced in CHO cells (chinese hamster ovary) and purified by standard chromatographic techniques. The glycoprotein encoded by erythropoietin (EPO) is synthesized in kidneys. It is the principal hormone that has a short half-life. Cells responsive to EPO have been identified in adult bone marrow, fetal liver, or adult spleen. Detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Solution, filtered through 0.2 μm pore size membrane. |
| Preparation Note: | Working solution: Dilute the concentrated EPO solution (250 U/ml) with PBS or culture medium containing 1 mg/ml (0.1%) BSA (or HSA) or 1 to 10% serum. Storage conditions (working solution): -15 to -25 °C Store the solution in aliquots at -15 to -25 °C Note: Avoid repeated freezing and thawing |
| Sequence: | One polypeptide chain (166 amino acids), glycosylated, identical to natural hEPO. The carbohydrate structure of recombinant EPO isolated from CHO cells is very similar to that of natural EPO. |
| Specificity: | Human Erythropoietin (hEPO) is effective on mouse and human cells. |
| Unit Definition: | EC50 definition: The amount of hEPO that is required to produce equivalent [3H]-thymidine incorporation into spleen cells from phenylhydrazine-treated mice to that expressed by 1 unit of the WHO-EPO reference standard (2nd IRP) (1 unit equals ≤10 ng). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| Purity | >98% (HPLC and SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |