cDNA Synthesis Kit
ROCHE/11117831001 - kit of for up to 10 reactions
Synonym: RT-PCR
Product Type: Chemical
| manufacturer/tradename | Roche |
| packaging | kit of for up to 10 reactions |
| Quality Level | 100  |
| Application: | Optimized one-tube procedure for the synthesis of double-stranded cDNA from total RNA or mRNA. The ds cDNA can be used for the construction of non-directional cDNA libraries, as a starting point for substractive hybridization experiments to enrich differentially expressed genes, and for the in vitro transcription of whole cDNA populations to generate labeled cRNA for hybridization on DNA microarrays. |
| Components: | • AMV Reverse Transcriptase Buffer, 5x concentrated • AMV Reverse Transcriptase (25 U/μl) • 1,4-Dithiothreithol (0.1 M) • Protector RNase Inhibitor (25 U/μl) • Oligo (dT)15 primer, 200 μM (1 μg/μl) • Oligo [(dT24 )T7promotor]65 primer, 100 μM (2 μg/μl) • dNTP mixture (each nucleotide, 10 mM) • Control neo mRNA (0.2 μg/μl) • 2nd strand synthesis buffer, 5x concentrated • 2nd strand enzyme blend (mixture of DNA Polymerase I, E. coli Ligase and RNase H) • T4 DNA Polymerase (1 U/μl) • Double-distilled water • RNase I (10 U/μl) • Proteinase K, recombinant, PCR grade (50 U/mll) |
| General description: | Optimized one-tube procedure for the synthesis of double-stranded cDNA up to 3 kb from total RNA or mRNA. |
| General description: | The cDNA Synthesis System uses an optimized one-tube procedure, based on the method of Gubler and Hoffmann. Sample: 1 - 20 μg total RNA, 0.3 - 2 μg mRNA Assay time: 60 minutes for 1st strand synthesis, 120 minutes for 2nd strand synthesis Product: double-stranded cDNA copy of an RNA sample |
| Other Notes: | Reverse Transcriptases Overview |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H319 - H334 |
| Precautionary statements | P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| UNSPSC | 41106313 |