BM-Condimed
ROCHE/10663573001 - suitable for cell culture, solution, pkg of 100 mL
Synonym: growth factor
Product Type: Chemical
| form | solution |
| manufacturer/tradename | Roche |
| packaging | pkg of 100 mL |
| Quality Level | 100  |
| solubility | water: miscible |
| sterility | 0.2 μm filtered |
| technique(s) | cell culture | mammalian: suitable |
| Application: | BM-Condimed is used as a supplement (10%, v/v) to the normal culture basal medium (e.g., F 10, M 199, RPMI 1640, DMEM), containing 10 to 20% FCS for the cultivation of human amniotic and chorionic villi cells. BM-Condimed was also shown to improve culture conditions for bone marrow cells and peripheral blood leukocytes. |
| General description: | BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Physiological solution, filtered through a 0.2 μm pore size membrane, buffered with sodium bicarbonate. |
| Preparation Note: | Working concentration: 1 to 100μg/ml 1 to 100μg/ml Working solution: Note: To 90ml of the normal basal medium F 10, M 199 Hanks, RPMI 1640, MEM Dulbecco, MEM Earle, Alpha MEM (containing 10 to 20% fetal bovine serum), add 10ml BM-Condimed. Do not use as a basal medium or as a replacement for serum. One percent L-glutamine may be added to the mixture. Biological activity: Each lot is assayed for its ability to stimulate the colony growth of primary amniotic cells. Source: BM-Condimed is prepared from the supernatant of human peripheral blood mononuclear cells stimulated with Con A. It contains a complex mixture of growth factors and lymphokines. Note: Do not add antibiotics, as these are already present in BM-Condimed, and additional antibiotics will affect the growth of the cells. |
| Storage and Stability: | Store at 2 to 8 °C. (Store in dark!) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | No data available |
| Flash Point(C) | No data available |
| UNSPSC | 12352207 |