Thrombin
ROCHE/10602400001 - from human plasma
Synonym: coagulation factor Iia; thrombin
Product Type: Chemical
| biological source | human plasma |
| form | lyophilized |
| manufacturer/tradename | Roche |
| mol wt | Mr ~33.6 kDa |
| optimum pH | 8.2-9.0 |
| packaging | pkg of 20 U |
| Quality Level | 100  |
| shipped in | wet ice |
| specific activity | ~120 units/mg protein (At 25 °C with Chromozym TH as the substrate.) |
| Application: | Thrombin has been used in the expression and purification of recombinant RELM (resistin-like moleculefamily members. It has also been used in platelet spreading on fibrinogen and immunostaining. |
| Application: | Thrombin is used in coagulation research, medical research, protein-structure analysis, and biochemical research. It has been used for the stimulation of the platelets. |
| Biochem/physiol Actions: | Thrombin plays an important role in blood coagulation. It serve as a procoagulant factor while transforming fibrinogen into an insoluble fibrin clot. Thrombin can act as anticoagulant by the activation of protein C. |
| General description: | Thrombin is a coagulation factor IIa. It is a serine endopeptidase that hydrolyzes peptide and ester bonds specifically at the carboxylic side of Arg. The enzyme converts fibrinogen to fibrin. The product contains EDTA and additional stabilizing agents. |
| General description: | Thrombin is a Na+ activated allosteric serine protease. It belongs to chymotrypsin family. Thrombin is made of two polypeptide chains of 36 (A chain) and 259 (B chain) residues that are covalently attached with a disulfide bond. |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Lyophilizate |
| Preparation Note: | Storage conditions (working solution): -15 to -25°C Inhibitors: DFP, TLCK, PMSF, benzamidine, α1-antitrypsin, α2-macroglobulin, antithrombin III heparin, hirudin, and APMSF |
| Reconstitution: | The reconstituted solution contains 20 mM EDTA. Important note: Use plastic vials and pipettes because thrombin will be adsorbed to glass surfaces! |
| Storage and Stability: | Store at 2 to 8 °C. (Store dry!) |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H332 |
| Precautionary statements | P261 - P271 - P304 + P340 + P312 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | does not flash |
| Flash Point(C) | does not flash |
| activity | specific activity: ~120 units/mg protein (At 25 °C with Chromozym TH as the substrate.) |
| Enzyme Commission (EC) Number | 3.4.21.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |